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SUPERKILLERTRAIL® Protein (soluble) (mouse), (recombinant)

ALX-201-130-3020 3x20 µg Inquire for pricing
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SUPERKILLERTRAIL® (soluble) (mouse), (recombinant) is an enhanced ligand that utilizes the Killer™ linker peptide mutated to increase disulfide-mediated cross-linking to form a more stable oligomer.

It has been well established that signals transmitted via TNF ligands and receptors are critical in mediating inflammation, cancer, and immunity. This has led to drug development aiming to either reduce or enhance receptor response. One limitation in this process is the inherent instability of recombinant ligands and their inability to effectively mimic the natural clustering of TNF receptors on the cell surface required for immune activation. Oligomerization of TNF ligands using enhancers such as SUPERKILLERTRAIL® has been shown to improve stability and significantly enhance immune activation compared to recombinant ligands alone.

Product Specification

Alternative Name:TNFSF 10, Apo-2L, CD253
Source:Produced in E. coli. The extracellular domain of mouse TRAIL (aa 99-291) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.
UniProt ID:P50591
Gene/Protein Identifier:A002862 (UCSD Signaling Gateway ID)
Formulation:Liquid. In 20mM HEPES, pH 7.4, 300mM sodium chloride, 0.01% Tween 20, 1% sucrose, 1mM DTT.
Purity:≥95% (SDS-PAGE)
Purity Detail:Affinity purified. Sterile filtered.
Endotoxin Content:<10.0EU/mg purified protein (LAL test; Bio Whittaker).
Specificity:Binds to mouse and less potently to human TRAIL receptors.
Biological Activity:Induces apoptosis at concentrations of >10ng/ml as tested on human tumor cells. This effect can be efficiently blocked by recombinant mouse TRAIL receptors (e.g. Prod. No. ALX-522-067 (rmTRAIL-R2:Fc)).
Note: This protein does not require a cross-linker (enhancer) for its potent biological activity.
Shipping:Shipped on Dry Ice
Long Term Storage:-80°C
Use/Stability:Once thawed it is recommended to prepare appropriate aliquots and to store them at -80°C. For a special storage and dilution buffer see Prod. No. ALX-505-005.
Handling:Avoid freeze/thaw cycles.
Scientific Background:TNF-related apoptosis-inducing ligand (TRAIL) is a protein ligand that induces the process of cell death called apoptosis.

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Product Literature References

N-glycosylation of mouse TRAIL-R restrains TRAIL-induced apoptosis: Y. Estornes, et al.; Cell Death Dis. 9, 494 (2018), Abstract; Full Text
Tumor necrosis factor-related apoptosis-inducing ligand mediates the resolution of allergic airway inflammation induced by chronic allergen inhalation: L. Faustino, et al.; Mucosal Immunol. 7, 1199 (2014), Abstract;
Loss of Caspase-9 Provides Genetic Evidence for the Type I/II Concept of CD95-mediated Apoptosis: A.K. Samraj, et al.; J. Biol. Chem. 281, 29652 (2006), Application(s): Death induction of J16, JMR, and F9 cells, Abstract; Full Text

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