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PTX3 (human), (recombinant)

 
ALX-201-124-C010 10 µg 379.00 USD
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Product Specification

Alternative Name:Pentraxin 3, TSG14, TNF stimulated gene-14
 
MW:~42kDa. Due to glycosylation, PTX3 may migrate between 40-45kDa.
 
Source:Produced in CHO cells. Human full-length PTX3.
 
UniProt ID:P26022
 
Concentration:0.1mg/ml.
 
Formulation:Liquid. In PBS containing 2% BSA and 0.1% sodium azide.
 
Applications:ELISA, WB
 
Application Notes:ELISA and Western blot standard together with MAb to PTX3 (Prod. No. ALX-804-464 (capture antibody)) and PAb to PTX3-Biotin (Prod. No. ALX-210-365B (detection antibody)).
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Handling:Avoid freeze/thaw cycles.
 
Protocol:Sandwich ELISA for PTX3
  • 96 well-ELISA plates (Nunc MaxiSorp 446612) were coated with 100µl of MAb to PTX3 (human) (MNB4) (Prod. No. ALX-804-464), 1μg/ml in 100µl coating buffer (15mM carbonate buffer pH 9.6) and incubated overnight at 4°C.
  • After incubation, the plates were washed three times with 300µl/well of washing buffer (PBS + 0.05% Tween 20), and then 300µl of 5% dry milk in washing buffer were added to block non-specific binding.
  • The plates were incubated for 2 hours at room temperature and then washed three times with washing buffer.
  • 50µl (in duplicate) recombinant human PTX3 standard (Prod. No. ALX-201-124) (75pg/ml to 2.4ng/ml ), cell supernatants or biological fluids diluted in PBS without Ca and Mg + 2% BSA were added and then incubated for 2 hours at 37°C.
  • The plates were washed five times with washing buffer, and then 100µl of PAb to PTX3 (human) (Biotin) (Prod. No. ALX-210-365B) 50ng/ml in washing buffer were added in each well.
  • The plates were incubated for 1 hour at 37°C and then washed five times with 300µl of washing buffer.
  • 100 µl/well streptavidin-horseradish peroxidase diluted 1:4’000 were added and the plates incubated for 1 hour at room temperature.
  • After incubation the plates were washed 5 times with washing buffer and 100µl of TMB substrate solution was added to each well. After incubation for 10 min. at room-temperature, 50μl of stop-solution (1M H2SO4) was added. Subsequently, absorbance was read within 30min at 450nm.

Note: Do not use heparin plasma or serum samples with this assay. EDTA samples are recommended. The detection value in serum is generally less than in plasma samples.

 

Product Literature References

Antitumor and anti-inflammatory effects of trabectedin on human myxoid liposarcoma cells: G. Germano, et al.; Cancer Res. 70, 2235 (2010), Abstract;
The long pentraxin Ptx3 is synthesized in IgA glomerulonephritis and activates mesangial cells: B. Bussolati, et al.; J. Immunol. 170, 1466 (2003), Abstract;
Long pentraxin PTX3 upregulates tissue factor expression in human endothelial cells: a novel link between vascular inflammation and clotting activation: E. Napoleone, et al.; Art. Thromb. Vasc. Biol. 22, 782 (2002), Abstract;
Non-redundant role of the long pentraxin PTX3 in anti-fungal innate immune response: C. Garlanda, et al.; Nature 420, 182 (2002), Abstract;
Circulating levels of the long pentraxin PTX3 correlate with severity of infection in critically ill patients: B. Muller, et al.; Crit. Care Med. 29, 1404 (2001), Abstract;
Dynamic induction of the long pentraxin PTX3 in the CNS after limbic seizures: evidence for a protective role in seizure-induced neurodegeneration: T. Ravizza, et al.; Neuroscience 105, 43 (2001), Abstract;
PTX3 in small-vessel vasculitides: an independent indicator of disease activity produced at sites of inflammation: F. Fazzini, et al.; Arthritis Rheum. 44, 2841 (2001), Abstract;
PTX3, A prototypical long pentraxin, is an early indicator of acute myocardial infarction in humans: G. Peri, et al.; Circulation 102, 636 (2000), Abstract; Full Text
The long pentraxin PTX3 binds to apoptotic cells and regulates their clearance by antigen-presenting dendritic cells: P. Rovere, et al.; Blood 96, 4300 (2000), Abstract; Full Text
Expression of a long pentraxin, PTX3, by monocytes exposed to the mycobacterial cell wall component lipoarabinomannan: V. Vouret-Craviari, et al.; Infect. and Immunity 65, 1345 (1997), Abstract;
Multimer Formation and Ligand Recognition by the Long Pentraxin PTX3. Similarities and differences with the short pentraxins C-reactive protein and serum amyloid P component: B. Bottazzi, et al.; J. Biol. Chem. 272, 32817 (1997), Abstract; Full Text

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