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KillerTRAIL™ (soluble) (human), (recombinant) (BULK)

 
ALX-201-123-C500 500 µg 1,442.00 USD
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Product Specification

Alternative Name:Apo-2L, TNFSF 10, CD253
 
Source:Produced in E. coli. The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide.
 
UniProt ID:P50591
 
Concentration:3mg/ml
 
Formulation:Liquid. In 20mM HEPES, pH 7.4, containing 150mM sodium chloride, 0.005% Tween 20, 1% sucrose, 1mM DTT.
 
Purity:≥98% (SDS-PAGE)
 
Purity Detail:Affinity purified.
 
Endotoxin Content:<10EU/µg protein (LAL test; Bio Whittaker).
 
Species reactivity:Human, Mouse
 
Specificity:Binds to human and mouse TRAIL receptors and osteoprotegerin (OPG).
 
Biological Activity:Induces apoptosis in a concentration range of 10-100ng/ml.
Note: This protein does not require a cross-linking enhancer for its potent biological activity. For cell lines that require extensive cross-linking of the TRAIL-Rs for killing (e.g. Jurkat) use SuperKillerTRAIL™ (Prod. No. ALX-201-115).
 
Specific Activity:ED50: 20ng/ml (BJAB cells)
 
Reconstitution:For a special storage and dilution buffer see KillerTRAIL™ Storage and Dilution Buffer (Prod. No. ALX-505-005).
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 
201-073-1
Figure: Schematic structure of human TRAIL (peptide aa 95-281).
201-073-2
Figure: Apoptosis of TRAIL-sensitive cells. Concentration dependence of apoptosis induction in Jurkat and BJAB cells by KillerTRAIL™, Soluble (human) (recombinant) (Prod. No. ALX-201-073) reveals high activity even at concentrations of 10-100ng/ml.Method: 5x104 human Jurkat (JK) or BJAB cells were cultured in 100µl RPMI 1640 medium containing 10% fetal calf serum for 16 hours at 37°C in a 96-well plate in the presence of the indicated concentrations of KillerTRAIL™. Concentrations of KillerTRAIL™ required to induce apoptosis may vary depending on the cell type studied. Cell death was quantified by forward/sideward scatter (FSC/SSC) combined with propidium iodide (PI) staining analysed by flow cytometry.
201-073-3
Figure: Specificity of KillerTRAIL™-induced apoptosis. Concentration dependent blocking of apoptosis induction of BJAB cells using MAb to TRAIL (human) (2E5) (Prod. No. ALX-804-296) reveals specificity of KillerTRAIL™-mediated killing.Method: Human BJAB cells were treated with KillerTRAIL™, Soluble (human) (recombinant) (at 300ng/ml) in the presence or absence of the indicated concentration of the MAb to TRAIL (human) (2E5) or a control IgG1 mouse monoclonal antibody. After 16 hours apoptosis was evaluated by forward/sideward scatter (FSC/SSC) combined with propidium iodide (PI) stain analysed by flow cytometry.
201-073-4
Figure: Apoptosis of TRAIL-sensitive myeloid cell line ML-1.
201-073-5
Figure: Flow cytometry analysis of the TRAIL-sensitive human leukemia cell line HL60 and of TRAIL-resistant K572 cells.
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Product Literature References

Differences and similarities in TRAIL-and tumor necrosis factor-mediated necroptotic signaling in cancer cells: J. Sosna, et al.; Mol. Cell. Biol. 36, 2626 (2016), Abstract;
The SMAC mimetic BV6 induces cell death and sensitizes different cell lines to TNF-α and TRAIL-induced apoptosis: M. El-Mesery, et al.; Exp. Biol. Med. (2016), Abstract;
Chronic lymphocytic leukemic cells exhibit apoptotic signaling via TRAIL-R1: M. MacFarlane, et al.; Cell Death Differ. 12, 773 (2005), Abstract;
TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors: M. Plasilova, et al.; Leukemia 16, 67 (2002), Abstract;
TRAIL and its receptors in the colonic epithelium: a putative role in the defense of viral infections: J. Sträter, et al.; Gastroenterology 122, 659 (2002), Abstract;
The anti-apoptotic protein BAG-3 is overexpressed in pancreatic cancer and induced by heat stress in pancreatic cancer cell lines: Q. Liao, et al.; FEBS Lett. 503, 151 (2001), Abstract;
The cytokines tumor necrosis factor-alpha (TNF-alpha ) and TNF-related apoptosis-inducing ligand differentially modulate proliferation and apoptotic pathways in human keratinocytes expressing the human papillomavirus-16 E7 oncoprotein: J.R. Basile, et al.; J. Biol. Chem. 276, 22522 (2001), Abstract; Full Text

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Produced in E. coli. The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge., ≥98% (SDS-PAGE). MS analysis, <1% impurity (mainly Hsp70 protein from E. coli) | Print as PDF
 
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