Product Details
| MW: | ~26kDa. |
| |
| Source: | Produced in E. coli. Active human granzyme K. |
| |
| EC: | 3.4.21.- |
| |
| UniProt ID: | P49863 |
| |
| Formulation: | Liquid. In PBS containing 10% sucrose and ~0.5M sodium chloride. Contains no preservatives. |
| |
| Purity: | ≥98% (SDS-PAGE) |
| |
| Specific Activity: | One unit is defined as the amount of enzyme that hydrolyzes 1nmol Z-Lys-SBzl per min. at 25ºC in 0.05M TRIS, pH 8.0, containing 0.15M NaCl, 0.01% Triton X-100 and 0.3mM DTNB. Specific activity provided in lot specific CoA. |
| |
| Shipping: | Dry Ice |
| |
| Long Term Storage: | -80°C |
| |
| Use/Stability: | Stable for at least 6 months when stored at -80°C. Dilute solutions (e. g. 1-1000ng/ml) should be used within 24 hours. |
| |
| Handling: | For maximum product recovery after thawing, centrifuge the vial before opening the cap. After opening, prepare aliquots and store at -80°C. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Product Literature References
Granzyme K+ CD8 T cells form a core population in inflamed human tissue: A.H. Jonsson, et al.; Sci. Transl. Med.
14, 649 (2022),
Application(s): Stimulation of primary fibroblasts in culture,
Abstract;
Full Text
Generation of catalytically active granzyme K from Escherichia coli inclusion bodies and identification of efficient granzyme K inhibitors in human plasma: E. Wilharm, et al.; J. Biol. Chem.
274, 27331 (1999),
Abstract;
Full Text
