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Granzyme K (human), (recombinant)

 
ALX-201-117-C010 10 µg 472.00 USD
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Product Specification

MW:~26kDa.
 
Source:Produced in E. coli. Active human granzyme K.
 
EC:3.4.21.-
 
UniProt ID:P49863
 
Concentration:0.2mg/ml
 
Formulation:Liquid. In PBS containing 10% sucrose and ~0.5M sodium chloride. Contains no preservatives.
 
Purity:≥98% (SDS-PAGE)
 
Specific Activity:~29U/µg protein. One unit is defined as the amount of enzyme that hydrolyzes 1nmol Z-Lys-SBzl per min. at 25°C in 0.05M TRIS, pH 8.0, containing 0.15M NaCl, 0.01% Triton X-100 and 0.3mM DTNB.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:Stable for at least 6 months when stored at -80°C. Dilute solutions (e. g. 1-1000ng/ml) should be used within 24 hours.
 
Handling:For maximum product recovery after thawing, centrifuge the vial before opening the cap. After opening, prepare aliquots and store at -80°C.
 

Product Literature References

Generation of catalytically active granzyme K from Escherichia coli inclusion bodies and identification of efficient granzyme K inhibitors in human plasma: E. Wilharm, et al.; J. Biol. Chem. 274, 27331 (1999), Abstract; Full Text

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