SUMO-1 activating enzyme (human), (recombinant)



ALX-201-090-C010	10 µg	164.00 USD

Activating enzyme for sumoylation assays.

Product Specification

Alternative Name:	SAE1/SAE2

Concentration:	0.2mg/ml

Formulation:	Liquid. In 50mM TRIS-HCl, pH 7.5, containing 1mM DTT and 200mM potassium chloride.

Source/Host:	Produced in E. coli. Heterodimer of SAE1 (aa 1-347) and SAE2 (aa 1-640).

Long Term Storage:	-80°C

Miscellaneous/General:	SAE1/SAE2 is a heterodimer that acts as the activating enzyme for SUMO-1, SUMO-2 and SUMO-3 and catalyzes formation of a thioester bond between the C-terminus of SUMO/sentrin (aa 1-347) and a cysteine residue in SAE2 (aa 1-640). In the presence of Ubc9, SAE1/SAE2 transfers the activated SUMO to a cysteine residue in Ubc9.

Background / Technical Information:	Standard reaction for protein conjugation (10µl) includes the target protein, SUMO-1 activating enzyme (Prod. No. ALX-201-090) (100ng), UbcH9 (Prod. No. ALX-201-046) (900ng), SUMO (10µg) in 50mM TRIS, pH 7.6, 5mM magnesium chloride, 2mM ATP, 10mM creatine phosphate, 3.5U/ml of creatine kinase and 0.6U/ml of inorganic pyrophosphatase. 10µg are sufficient for about 100 assays. UniProt ID Q9UBE0: SUMO-1 Activating Enzyme Subunit 1 (human) UniProt ID O95605: SUMO-1 Activating Enzyme Subunit 2 (human)

Product Literature References

Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9: M.H. Tatham, et al.; J. Biol. Chem. 276, 35368 (2001), Abstract; Full Text

Protein modification by SUMO: R.T. Hay; TIBS 26, 332 (2001), Abstract;

Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1: J.M. Desterro, et al.; J. Biol. Chem. 274, 10618 (1999), Abstract; Full Text