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SUMO-2 (human) (1-93), (recombinant)

Active form of SUMO-2
 
ALX-201-089-C500 500 µg 274.00 USD
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Active form of human SUMO-2 for conjugation to protein substrates.

Product Specification

Alternative Name:Sentrin-2, Small ubiquitin-related modifier 2, SUMO-3
 
Source:Produced in E. coli.
 
UniProt ID:P61956
 
Formulation:Liquid. In PBS.
 
Purity:≥95%
 
Application Notes:Standard reaction for protein conjugation (10µl) includes the target protein, SUMO-1 activating enzyme (Prod. No. BML-UW9330) (100ng), UbcH9 (900ng) (Prod. No. ALX-201-046), SUMO-2 (1-93) (10µg) in 50mM TRIS, pH 7.6, 5mM magnesium chloride, 2mM ATP, 10mM creatine phosphate, 3.5U/ml of creatine kinase and 0.6U/ml of inorganic pyrophosphatase.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-80°C
 
Use/Stability:Stable for a few days at room temperature.  
 
Technical Info/Product Notes:Formerly this product was called SUMO-3 (1-93) (human) (recombinant), but the name was changed in accordance with UniProt.
 

Product Literature References

Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9: M.H. Tatham, et al.; J. Biol. Chem. 276, 35368 (2001), Abstract; Full Text
Protein modification by SUMO: R.T. Hay; TIBS 26, 332 (2001), Abstract;
Ubch9 conjugates SUMO but not ubiquitin: J.M. Desterro, et al.; FEBS Lett. 417, 297 (1997), Abstract;

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