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PARP-2 (mouse), (recombinant) (high purity)

 
ALX-201-064-C020 20 µg 533.00 USD
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Product Specification

Alternative Name:Poly(ADP-ribose) polymerase 2
 
Source:Produced in Sf9 cells (full length).
 
EC:2.4.2.30
 
UniProt ID:O88554
 
Concentration:~1mg/ml
 
Formulation:Liquid. In 50mM TRIS-HCl, pH 7.5, containing 1mM DTT, 0.5mM EDTA, 0.5mM PMSF and 20% glycerol.
 
Purity:≥98% (SDS-PAGE)
 
Purity Detail:Affinity purified.
 
Specific Activity:≥200U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1nmol of poly(ADP-ribose) per min at 25°C, pH 7.5. See also separate protocol (link below).
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles.
 
201-064 new
Quality Control: 12% SDS-Polyacrylamide gel.
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201-064 new

Product Literature References

Poly(ADP-ribose) reactivates stalled DNA topoisomerase I and Induces DNA strand break resealing: M. Malanga & F.R. Althaus; J. Biol. Chem. 279, 5244 (2004), Abstract; Full Text
A bidirectional promoter connects the poly(ADP-ribose) polymerase 2 (PARP-2) gene to the gene for RNase P RNA. structure and expression of the mouse PARP-2 gene: J.C. Ame, et al.; J. Biol. Chem. 276, 11092 (2001), Abstract; Full Text
PARP-2, A novel mammalian DNA damage-dependent poly(ADP-ribose) polymerase: J.C. Ame, et al.; J. Biol. Chem. 274, 17860 (1999), Abstract; Full Text

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