Product Details
| Alternative Name: | Poly(ADP-ribose) polymerase 1 |
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| Source: | Produced in Sf9 insect cells. Produced in a baculovirus expression system. |
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| EC: | 2.4.2.30 |
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| UniProt ID: | P09874 |
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| Concentration: | 1mg/ml |
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| Formulation: | Liquid. In 100mM TRIS-HCl, pH 7.5, containing 14mM β-mercaptoethanol, 0.5mM EDTA, 0.5mM PMSF and 10% glycerol. |
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| Purity: | ≥99% (SDS-PAGE) |
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| Purity Detail: | Affinity purified. |
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| Specific Activity: | ≥600U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1nmol of poly(ADP-ribose) per min. at 25°C, pH 7.5. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Handling: | Avoid freeze/thaw cycles. |
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| Regulatory Status: | RUO - Research Use Only |
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Quality Control: 10% SDS-Polyacrylamide gel.
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Product Literature References
TET-mediated DNA hydroxymethylation is negatively influenced by the PARP-dependent PARylation: A. Tolić , et al.; Epigenetics Chromatin
15, 11 (2022),
Abstract;
Identification by Inverse Virtual Screening of magnolol-based scaffold as new tankyrase-2 inhibitors: S. Di Micco, et al.; Bioorg. Med. Chem.
26, 3953 (2018),
Abstract;
Mechanistic Insights into Autoinhibition of the Oncogenic Chromatin Remodeler ALC1: L.C. Lehmann, et al.; Mol. Cell
68, 847 (2017),
Abstract;
Full Text
PARP1-driven poly-ADP-ribosylation regulates BRCA1 function in homologous recombination-mediated DNA repair: Y. Hu, et al.; Cancer Discov.
4, 1430 (2014),
Abstract;
Full Text
A family of macrodomain proteins reverses cellular mono-ADP-ribosylation: G. Jankevicius, et al.; Nat. Struct. Mol. Biol.
20, 508 (2013),
Abstract;
Mapping PARP-1 auto-ADP-ribosylation sites by liquid chromatography-tandem mass spectrometry: J.D. Chapman, et al.; J. Proteome Res.
12, 1868 (2013),
Abstract;
Poly(ADP-ribose) polymerase-1 modulates Nrf2-dependent transcription: T. Wu, et al.; Free Radic. Biol. Med.
67C, 69 (2013),
Abstract;
Regulation of poly(ADP-ribose) polymerase-1 functions by leukocyte elastase inhibitor/LEI-derived DNase II during caspase-independent apoptosis: C. Leptrête, et al.; Int. J. Biochem. Cell Biol.
41, 1046 (2009),
Abstract;
Poly(ADP-ribose) polymerase 1 binds to Pax8 and inhibits its transcriptional activity: T. Di Palma, et al.; J. Mol. Endocrinol.
41, 379 (2008),
Abstract;
Poly(ADP-ribose) reactivates stalled DNA topoisomerase I and Induces DNA strand break resealing: M. Malanga & F.R. Althaus; J. Biol. Chem.
279, 5244 (2004),
Abstract;
Full Text
Overproduction and large-scale purification of the human poly(ADP- ribose) polymerase using a baculovirus expression system: H. Giner, et al.; Gene
114, 279 (1992),
Abstract;
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