Product Details
| Alternative Name: | FLICE, Mch5, MACH |
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| Source: | Produced in E. coli. Contains an N-terminal His-tag. |
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| EC: | 3.4.22.- |
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| UniProt ID: | Q14790 |
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| Formulation: | Lyophilized. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Specific Activity: | 5’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate IETD-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM sodium chloride, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT. |
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| Application Notes: | Useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates or as positive control in caspase activity assays. We recommend using 1U per assay for analyzing caspase activity. |
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| Reconstitution: | Reconstitute to 1U/µl with PBS containing 15% glycerol. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C. |
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| Scientific Background: | Caspase-8 is a member of the interleukin-1β converting enzyme (ICE) family of cysteine proteases. Same as other caspases, caspase-7 also exists in cells as an inactive proenzyme. During apoptosis procaspase-8 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed form of caspase-8 consists of large and small subunits which associate to form the active enzyme. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Active human caspase was expressed in E. coli and purified. The activity of recombinant caspase-8 was determined by cleaving AFC conjugates of IETD. The cleavage activity was effectively inhibited by the corresponding peptide inhibitor as indicated.
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Product Literature References
GSDMEa-mediated pyroptosis is bi-directionally regulated by caspase and required for effective bacterial clearance in teleost: H. Xu, et al.; Cell Death Dis.
13, 491 (2022),
Abstract;
The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8: J. Zhang, et al.; Cell Res.
31, 980 (2021),
Abstract;
RIPK1 and Caspase-8 Ensure Chromosome Stability Independently of Their Role in Cell Death and Inflammation: G. Liccardi, et al.; Mol. Cell
73, 413 (2019),
Abstract;
Full Text
Tom20 senses iron-activated ROS signaling to promote melanoma cell pyroptosis: B. Zhou, et al.; Cell Res.
28, 1171 (2018),
Abstract;
Full Text
Pyroptosis and apoptosis pathways engage in bidirectional crosstalk in monocytes and macrophages: C.Y. Taabazuing, et al.; Cell Chem. Biol.
24, 507 (2017),
Abstract;
Full Text
