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Caspase-9 (human), (recombinant) (active)

ALX-201-047-U025 25 U 295.00 USD
ALX-201-047-U100 100 U 458.00 USD
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Product Specification

Alternative Name:ICE-LAP-6, Mch6
Source:Produced in E. coli. Contains an N-terminal His-tag.
UniProt ID:P55211
Purity:≥90% (SDS-PAGE)
Specific Activity:~400U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate LEHD-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
Application Notes:Useful in screening caspase inhibitors, studying enzyme regulation, determining target substrates or as positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
Reconstitution:Reconstitute to 1U/µl with 25µl or 100µl PBS containing 15% glycerol.
Note: It is normal to see some insoluble protein following reconstitution. This is mainly due to denaturation of small amounts of protein during preparation. Precipitate should not affect activity. If preferred, remove precipitate by brief centrifugation.
Shipping:Shipped on Dry Ice
Long Term Storage:-80°C
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Scientific Background:Caspase-9 (also known as Ice-Lap6 and Mch6) is a member of the interleukin-1b converting enzyme (ICE) family of cysteine proteases. Similar as other caspases, caspase-9 also exists in cells as an inactive proenzyme. During the initiation of apoptosis procaspase-9 is processed at aspartate residues to form active caspase-9. As one of the initiator caspases, active caspase-9 functions to trigger the activation of downstream effector caspases, leading to disassembly of cell structures. The active recombinant caspase-9 is routinely tested for its ability to enzymatically cleave the caspase-9 colorimetric substrate Ac-LEHD-pNA (Prod. No. ALX-260-081).

Product Literature References

Differential apoptosis-related protein expression, mitochondrial properties, proteolytic enzyme activity, and DNA fragmentation between skeletal muscles: E.M. McMillan, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 300, R531 (2011), Abstract; Full Text
A conserved XIAP-interaction motif in caspase-9 and Smac/DIABLO regulates caspase activity and apoptosis: S.M. Srinivasula, et al.; Nature 410, 112 (2001), Abstract;
Caspase-9, Bcl-XL, and Apaf-1 form a ternary complex: G. Pan, et al.; J. Biol. Chem. 273, 5841 (1998), Abstract; Full Text
Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade: P. Li, et al.; Cell 91, 479 (1997), Abstract;
ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B: H. Duan, et al.; J. Biol. Chem. 271, 16720 (1996), Abstract; Full Text
The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32: S.M. Srinivasula, et al.; J. Biol. Chem. 271, 27099 (1996), Abstract; Full Text

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