United States
UbcH9 (recombinant)
Conjugating enzyme (E2)
| ALX-201-046-C050 | 50 µg | 202.00 USD |  |
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Product Specification
| Alternative Name: | Ubc9 (human) |
| Concentration: | 1.2mg/ml |
| Purity: | ≥95% |
| Formulation: | Liquid. In 50mM TRIS-HCl, pH 7.5, containing 5mM β-mercaptoethanol. |
| EC: | 6.3.2.19 |
| Source/Host: | Produced in E. coli. |
| Application: | UbcH9 catalyzes formation of an isopeptide bond between the C-terminus of SUMO-1/sentrin-1 and ε-amino groups in the target protein. |
| Long Term Storage: | -80°C |
| Background / Technical Information: | Standard reaction for protein conjugation (10µl) includes the target protein, SUMO-1 activating enzyme (Prod. No. ALX-201-090) (100ng), UbcH9 (900ng) (Prod. No. ALX-201-046), SUMO-1 (1-97) (Prod. No. ALX-201-045) (10µg) in 50mM TRIS, pH 7.6, 5mM magnesium chloride, 2mM ATP, 10mM creatine phosphate, 3.5U/ml of creatine kinase and 0.6U/ml of inorganic pyrophosphatase. UniProt ID P50550: Ubc9 (human) AfCS Signaling Gateway ID A002350: Ubc9 (mouse) |
Product Literature References
SUMO-1 modification activates the transcriptional response of p53: M.S. Rodriguez, et al.; EMBO J. 18, 6455 (1999), Abstract; Full Text
SUMO-1 modification of IkappaBalpha inhibits NF-kappaB activation: J.M. Desterro, et al.; Mol. Cell 2, 233 (1998), Abstract;
Ubch9 conjugates SUMO but not ubiquitin: J.M. Desterro, et al.; FEBS Lett. 417, 297 (1997), Abstract;
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