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Nitric oxide synthase (neuronal) (rat), (recombinant) (high purity)

 
ALX-201-028-R050 50 µl 159.00 USD
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Replaces Prod. #: BML-SE126

Product Specification

Alternative Name:nNOS, NOS I
 
MW:~160kDa/subunit; homodimer.
 
Source:Produced in Sf9 insect cells. Produced in a baculovirus expression system.
 
EC:1.14.13.39
 
UniProt ID:P29476
 
Concentration:~1mg/ml
 
Formulation:Liquid. In 20mM TRIS/HCl (pH 7.5), 150mM sodium chloride, 4mM EGTA and 10mM 2-mercaptoethanol.
 
Purity:≥98% (SDS-PAGE)
 
Specific Activity:~0.5µmol L-citrulline/mg/min. Amount assayed: 0.1-0.3µl. Optimal conditions: pH 7.0; 37°C; 0.1mM L-arginine, 0.2mM NADPH, 10µM BH4, 5µM FAD/FMN, 10µg/ml calmodulin, 10-500µM CaCl2.
 
Application Notes:Useful tool for studying NOS enzyme regulation, screening inhibitors and other applications.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:Dilute stock solutions with buffer containing 10mM 2-mercaptoethanol and 1mM CHAPS.

nNOS is relatively unstable. Keep stock vial on ice at all times when performing experiments! Enzyme loses approx. 10-15% of activity during a single freeze/thaw cycle.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 

Product Literature References

Calmodulin controls neuronal nitric-oxide synthase by a dual mechanism. Activation of intra- and interdomain electron transfer: H.M. Abu-Soud, et al.; J. Biol. Chem. 269, 32047 (1994), Abstract; Full Text
Characterization of neuronal nitric oxide synthase and a C415H mutant, purified from a baculovirus overexpression system: M.K. Richards & M.A. Marletta; Biochemistry 33, 14723 (1994), Abstract;
Electron transfer in the nitric-oxide synthases. Characterization of L- arginine analogs that block heme iron reduction: H.M. Abu-Soud, et al.; J. Biol. Chem. 269, 32318 (1994), Abstract; Full Text
Evidence for a bidomain structure of constitutive cerebellar nitric oxide synthase: E.A. Sheta, et al.; J. Biol. Chem. 269, 15147 (1994), Abstract; Full Text
Expression of rat brain nitric oxide synthase in baculovirus-infected insect cells and characterization of the purified enzyme: C. Harteneck, et al.; Biochem. J. 304, 683 (1994), Abstract;
Molecular mechanisms of inhibition of porcine brain nitric oxide synthase by the antinociceptive drug 7-nitro-indazole [published erratum appears in Neuropharmacology 1995 Feb;34(2):243]: B. Mayer, et al.; Neuropharmacology 33, 1253 (1994), Abstract;
Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme: D.S. Bredt & S.H. Snyder; PNAS 87, 682 (1990), Abstract;

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