Specific inhibitor of matrix metalloproteinases (MMPs). It has no activity on mammalian membrane metalloproteinases, procollagen peptidase or on bacterial metalloproteinases such as thermolysin.
Product Details
| Alternative Name: | Tissue inhibitor of metalloproteinase 1 |
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| MW: | ~28kDa. |
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| Source: | Isolated from stimulated human neutrophils. The secreted protein consists of 184 amino acids, six disulfide bonds and two glycosylation sites containing N-linked oligosaccharides. |
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| UniProt ID: | P01033 |
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| Formulation: | Liquid. In 50mM TRIS-HCl, pH 7.0, containing 200mM sodium chloride, 5mM CaCl2, 1µM ZnCl2, 0.05% BRIJ-35 and 0.05% sodium azide. |
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| Purity: | ≥92% (SDS-PAGE, Western blot) |
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| Shipping: | Dry Ice |
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| Short Term Storage: | -20°C |
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| Long Term Storage: | -80°C |
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| Use/Stability: | TIMP-1 is very stable if stored at -80°C. It can be kept at -20°C for several months or +4°C for weeks without significant loss of activity. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Technical Info/Product Notes: | Note: It is recommended to perform a preincubation of ~20 min. at 37°C to allow formation of the enzyme-inhibitor complex. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Shedding of the Transferrin Receptor Is Mediated Constitutively by an Integral Membrane Metalloprotease Sensitive to Tumor Necrosis Factor alpha Protease Inhibitor-2: M. Kaup, et al.; J. Biol. Chem.
277, 38494 (2002),
Abstract;
Full Text
Progelatinase B forms from human neutrophils. complex formation of monomer/lipocalin with TIMP-1: H. Kolkenbrock, et al.; Biol. Chem.
377, 529 (1996),
Abstract;
Generation and activity of the ternary gelatinase B/TIMP-1/LMW-stromelysin-1 complex: H. Kolkenbrock, et al.; Biol. Chem.
376, 495 (1995),
Abstract;
Tissue inhibitor of metalloproteinase (TIMP-2). A new member of the metalloproteinase inhibitor family: W.G. Stettler-Stevenson, et al.; J. Biol. Chem.
264, 17374 (1989),
Abstract;
Full Text
Stromelysin is an activator of procollagenase. A study with natural and recombinant enzymes: G. Murphy, et al.; Biochem. J.
248, 265 (1987),
Abstract;
The interaction of purified rabbit bone collagenase with purified rabbit bone metalloproteinase inhibitor: T.E. Cawston, et al.; Biochem. J.
211, 313 (1983),
Abstract;
