Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

MMP-8 proenzyme (human neutrophils)

 
ALX-200-421-C005 5 µg 121.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:Matrix metalloproteinase 8, Neutrophil collagenase, Collagenase-2
 
MW:~85kDa.
 
Source:Isolated from stimulated human neutrophil granulocytes (buffy coat). Requires activation.
 
EC:3.4.24.34
 
UniProt ID:P22894
 
Concentration:100µg/ml
 
Formulation:Liquid. In 50mM TRIS-HCl, pH 7.0, containing 200mM NaCl, 5mM CaCl2, 1µM ZnCl2, 0.05% BRIJ 35, and 0.05% sodium azide.
 
Purity:≥90% (SDS-PAGE)
 
Specific Activity:≥60mU/mg protein (Y. Masui, et al.; Biochem. Med. 17, 215 (1977)). One unit is defined as the amount of enzyme that hydrolyzes 1µmol 2,4-dinitrophenyl-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min. at 37°C, pH 7.0.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:Stable for 1 week when stored at +4°C and for serveral weeks when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:MMP-8 is a glycoprotein containing complex N-linked oligosaccharides. It hydrolyzes type I over type II, and III collagens. Activated MMP-8 is inhibited by TIMP-1 (Prod. No. ALX-200-426).
 
Technical Info/Product Notes:Precursor enzyme needs activation using 2mM AMPA (aminophenylmercuric acetate) or 1mM mersalylic acid for 60 min. at 37°C. Alternatively use 0.1mM PCMB (p-chloromercuribenzoate) or 10µg/ml trypsin for 20 min. at 25°C; PCMB is substantially more effective.
 

Product Literature References

A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases: C.G. Knight, et al.; FEBS Lett. 296, 263 (1992), Abstract;
Characterization of 58-kilodalton human neutrophil collagenase: comparison with human fibroblast collagenase: S.K. Mallya, et al.; Biochemistry 29, 10628 (1990), Abstract;
Secreted forms of human neutrophil collagenase: K.A. Hasty, et al.; J. Biol. Chem. 261, 5645 (1986), Abstract; Full Text
Synthetic substrates for vertebrate collagenase: Y. Masui, et al.; Biochem. Med. 17, 215 (1977), Abstract;

Related Products

TIMP-1 (human neutrophils) 

Isolated from stimulated human neutrophils. The secreted protein consists of 184 amino acids, six disulfide bonds and two glycosylation sites containing N-linked oligosaccharides., ≥92% (SDS-PAGE, Western blot) | Print as PDF
 
ALX-200-426-C005 5 µg 283.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Print as PDF
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
MMP
MMP-8
By biological activity:
MMP Native protein
MMP Enzyme
MMP-8 Native protein
MMP-8 Enzyme
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Technical Posters
Disease-Associated Stress Signaling
Disease-Associated Stress Signaling
Download as PDF

All new literature pieces

Recommend this page

 
Keep in touch

©2017 Enzo Life Sciences, Inc.,