Control peptide for D-JNKI.
|Alternative Name:||JNK Inhibitor 1 (D-stereoisomer) control peptide|
|Formulation:||Liquid. In sterile PBS.|
|Shipping:||Shipped on Blue Ice|
|Long Term Storage:||-20°C|
|Handling:||Keep on ice at all times.|
|Technical Info/Product Notes:||Note: D-TAT consists of D-amino acids. The sequence of unconjugated D-TAT is retro-inverse compared to L-TAT.|
Inhibition of c-Jun phosphorylation by synthetic L-JNKI1 (Prod. No. ALX-159-600
) in vitro
Kinase assays with recombinant JNK1α1 enzyme produced in a reticulocyte lysate system (lanes 2-4) in the presence or absence of L-JNKI1 or TAT peptides (20µM final concentration) were performed as described . L-TAT peptide (Prod. No. ALX-168-009
) serves as control. The degree of JNK inhibition is determined by autoradiography of 32
P-labelled c-Jun (aa 1-89) (upper panel). Coomassie staining shows that equal amounts of the c-Jun substrate were applied in all lanes (lower panel).Note:
Inhibition by D-JNKI1 (Prod. No. ALX-159-601) requires higher concentrations in this in vitro
assay. Minus indicates no peptide added. Signal in the first lane without the recombinant JNK1a1 added results from JNK background activity in the reticulocyte lysate that was used to produce JNK1a1.
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