Sirtuin 2 (human) (IntraCellular) ELISA Kit



AG-45A-0030EK-KI01	96 wells		660.00 USD

AG-45A-0030TP-KI01	2x96 wells	Twin Plex	950.00 USD

This kit is an enzyme-linked immunosorbent assay (ELISA) used for the quantitative determination of intracellular sirtuin 2 in human cell lysates.

Product Specification

Alternative Name:	Sirt2, SIR2-like Protein 2

Sensitivity:	80pg/ml (range 0.125 to 8ng/ml)

Specificity:	Does not cross-react with human sirtuin 1, 5, and 6.

Application:	For the quantitative determination of intracellular sirtuin 2 in human cell lysates.

Long Term Storage:	+4°C

Use/Stability:	Diluted wash solution may be stored at room temperature for up to one month.

Miscellaneous/General:	Sirtuin 2 is a NAD-dependent histone deacetylase whose size is a calculated molecular mass of 39.5 kD. It is largely localized at the cytoplasm where it is physically associated with microtubules. SIRT2 was found to deacetylate lys⁴⁰ of alpha-tubulin both in vitro and in vivo. SIRT2 co-localizes and interacts in vivo with HDAC6, another tubulin deacetylase. Enzymatic analysis of recombinant SIRT2 in comparison to a yeast homolog of Sir2 protein shows a striking preference of SIRT2 for acetylated tubulin peptide as a substrate relative to acetylated histone H3 peptide. These observations established SIRT2 as a bona fide tubulin deacetylase. It has also been shown that SIRT2 is a regulator of mitotic progression that acts downstream from CDC14B in a pathway regulating mitotic exit or subsequent cytokinesis. Inhibition of SIRT2 rescues alpha-synuclein toxicity and modifies inclusion morphology in a cellular model of Parkinson disease. Genetic inhibition of SIRT2 via small interfering RNA similarly rescued alpha-synuclein toxicity, suggesting a link between neurodegeneration and aging via SIRT2.

Background / Technical Information:	UniProt ID Q8IXJ6: SIRT2 (human)

Manufacturer:	Manufactured by AdipoGen, Inc.