The Nampt (Visfatin/PBEF) (human) Intracellular ELISA Kit is a complete assay for the quantitative determination of human Nampt (visfatin/PBEF) levels. This kit is to be used with cell lysates from human origin.
Product Details
| Alternative Name: | Nicotinamide Phosphoribosyltransferase, Pre-B-Cell Colony Enhancing Factor 1 |
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| Sensitivity: | 30pg/ml (range 0.25 – 16 ng Nampt/ml) |
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| Applications: | ELISA
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| Application Notes: | For the quantitative determination of human Nampt (visfatin/PBEF) in cell lysates. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Human
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| Specificity: | Has been shown to specifically detect the physiologically relevant Nampt/visfatin dimer in human serum samples. |
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| Crossreactivity: | Does not cross-react with human adiponectin, human resistin, human RELM-β, human leptin, human GPX3, human ANGPTL4, human FABP4, human ANGPTL6, human PAI1. Shows 5% cross-reactivity with mouse Nampt and 15% cross reactivity with rat Nampt. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | +4°C |
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| Technical Info/Product Notes: | Cited samples:
Cytokine ELISAs Cited Samples |
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| UniProt ID: | P43490 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Aerobic and resistance exercise training reverses age-dependent decline in NAD+ salvage capacity in human skeletal muscle: R.M. de Guia, et al.; Physiol. Rep.
7, e14139 (2019),
Application(s): ELISA using human plasma,
Abstract;
Full Text
NADP+ is an endogenous PARP inhibitor in DNA damage response and tumor suppression: C. Bian, et al.; Nat. Commun.
10, 693 (2019),
Application(s): ELISA using cell lysates,
Abstract;
Full Text
Ethnic differences in the role of adipocytokines linking abdominal adiposity and insulin sensitivity among asians: E. Parvaresh Rizi, et al.; J. Clin. Endocrinol. Metab.
100, 4249 (2015),
Application(s): ELISA using human plasma,
Abstract;
Visfatin in obese children and adolescents and its association with insulin resistance and metabolic syndrome: M. Nourbakhsh, et al.; Scand. J. Clin. Lab. Invest.
75, 183 (2015),
Application(s): ELISA using human serum,
Abstract;
Diurnal rhythm of circulating nicotinamide phosphoribosyltransferase (Nampt/visfatin/PBEF): impact of sleep loss and relation to glucose metabolism: C. Benedict, et al.; J. Clin. Endocrinol. Metab.
97, E218 (2012),
Application(s): ELISA using human serum,
Abstract;
Correlation of circulating full-length visfatin (PBEF/Nampt) with metabolic parameters in subjects with and without diabetes: a cross-sectional study: R. Retnakaran, et al.; Clin. Endocrinol. (Oxf)
69, 885 (2008),
Abstract;
Serum levels of the adipokine visfatin are increased in pre-eclampsia: M. Fasshauer, et al.; Clin. Endocrinol. (Oxf)
69, 69 (2008),
Abstract;
Molecular characteristics of serum visfatin and differential detection by immunoassays: A. Körner, et al.; J. Clin. Endocrinol. Metab.
92, 4783 (2007),
Abstract;
Full Text
Visfatin is present in bovine mammary epithelial cells, lactating mammary gland and milk, and its expression is regulated by cAMP pathway: T. Yonezawa, et al.; FEBS Lett.
580, 6635 (2006),
Abstract;
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