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UGGT polyclonal antibody

 
ADI-VAP-PT068-E 100 µl 282.00 USD
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Product Specification

Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to a portion of rat UGGT.
 
UniProt ID:Q9JLA3
 
Species reactivity:Mouse, Rat
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1:100 colorimetric, 1:500 ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~170kDa by Western blot.
 
Formulation:Liquid. Whole rabbit serum.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:UDP-glucose:glycoprotein glucosyltransferase (UGGT) is a 170 kDa lumenal endoplasmic reticulum (ER) resident protein that catalyzes monoglucosylation of high mannose oligosaccharides. UGGT catalyzes the transfer of glucose from UDP-glucose to Man7-9GlcNAc2-moiety of unfolded glycoproteins in a Ca2+ dependent reaction. UGGT binds unfolded glycoprotein via an N-acetylglucosamine unit on the oligosaccharide, and hydrophobic amino acids that are exposed in the denatured conformation.
 
UGGT polyclonal antibody Western blot
Western blot analysis of rat brain tissue extract, probed with UGGT, pAb.
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UGGT polyclonal antibody Western blot

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