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Dynamin monoclonal antibody (D5)

 
ADI-VAM-SV041-E 100 µg 306.00 USD
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Product Specification

Alternative Name:DNM, DYN
 
Clone:D5
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant His6-tagged human dynamin.
 
UniProt ID:Q05193 (Dynamin-1), P50570 (Dynamin-2), Q9UQ16 (Dynamin-3)
 
Source:Purified from ascites.
 
Species reactivity:Human, Mouse, Rat
Bovine, Chicken
 
Applications:Flow Cytometry, IHC, IP, WB
 
Recommended Dilutions/Conditions:Flow Cytometry (10µg/ml)
Immunohistochemistry (1:50, human colon cancer tissue)
Western Blot (0.5µg/ml, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~100kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 0.1mM PMSF and 50% glycerol.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Dynamin is a 100 kDa GTP binding protein that was first identified as a protein able to bind microtubules. To date, three genes have been found to encode Dynamin isoforms. Dynamin 1 is neuronal specific, Dynamin 2 is ubiquitously expressed and Dynamin 3 localizes to testis, brain and lung. Dynamin may mediate vesicle formation by assembling into a ring structure around the necks of invaginated coated pits that may eventually lead to the closure of the neck and formation of vesicles.
 
Dynamin monoclonal antibody (D5) Western blot
Dynamin monoclonal antibody (D5) Western blot
Western blot analysis of Dynamin, mAb (D5) (Prod. No. ADI-VAM-SV041): Lane 1: MW marker, Lane 2: Mouse Brain (Prod. No.: ADI-LYT-MB100), Lane 3: Rat Brain (Prod. No.: ADI-LYT-RB100)
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Dynamin monoclonal antibody (D5) Western blot Dynamin monoclonal antibody (D5) Western blot

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