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SNAP α/β monoclonal antibody (6B7-3)

ADI-VAM-SV027-C 25 µg 110.00 USD
ADI-VAM-SV027-E 100 µg 282.00 USD
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Product Specification

Alternative Name:Soluble NSF attachment protein α/β
Immunogen:Recombinant bovine α-SNAP.
UniProt ID:P81125
Species reactivity:Human, Mouse, Rat
Bovine, Dog, Hamster, Sheep
Applications:IHC (PS), IP, WB
Recommended Dilutions/Conditions:Western Blot (1.5µg/ml, colorimetric, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~35kDa by Western blot.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 0.1mM PMSF and 50% glycerol.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Synaptic vesicle fusion reactions are mediated by a variety of SNAP proteins, including alpha and beta SNAPs and SNAP-25. Together with synaptotagmin and NSF, alpha-SNAP modulates the interaction of SNAP25, VAMP, and syntaxin in an ATP-dependent manner to form a 20S complex associated with synaptic vesicle docking and fusion. beta-SNAP interacts with synaptotagmin and may be involved in calcium-regulated exocytosis.
Immunohistochemistry analysis of human brain, cortex tissue stained with SNAP α/β, mAb (6B7-3) at 10µg/ml.
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Product Literature References

Cellubrevin alterations and Mycobacterium tuberculosis phagosome maturation arrest: V. Deretic, et al. ; J. Biol. Chem. 277, 17320 (2002), Application(s): WB using mouse samples, Abstract;
Differential binding of traffic-related proteins to phosphatidic acid- or phosphatidylinositol (4,5)- bisphosphate-coupled affinity reagents: N.T. Ktistakis, et al. ; J. Biol. Chem. 276, 8987 (2001), Application(s): WB using sheep samples, Abstract;
Role of phosphatidylinositol 3-kinase and Rab5 effectors in phagosomal biogenesis and mycobacterial phagosome maturation arrest: V. Deretic, et al. ; J. Cell Biol. 154, 631 (2001), Application(s): WB using mouse samples, Abstract;

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