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rSec6 monoclonal antibody (9H5)

 
ADI-VAM-SV021-D 50 µg 194.00 USD
 
ADI-VAM-SV021-F 200 µg 399.00 USD
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Product Specification

Alternative Name:Exocyst complex component 3
 
Clone:9H5
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Recombinant rat Sec6.
 
UniProt ID:Q62825
 
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Dog, Hamster, Monkey, Porcine, Rabbit, Sheep
 
Applications:ICC, IHC (PS), WB
 
Recommended Dilutions/Conditions:Western Blot (1:250, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~87kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:rSec6 and rSec8 are rat homologs of the yeast Sec6 and Sec8 proteins that are essential for protein transport between the Golgi apparatus and the plasma membrane. In yeast, Sec8 is part of a 19.5S complex that includes Sec6 and Sec15, that also associate with the ras-like GTP binding protein Sec4. An analogous 17S complex in rat brain was found to contain rSec6 and rSec8, along with eight proteins with a combined molecular weight of 743 kDa. The membrane-associated rSec6/8 complex co-immunoprecipitates with syntaxin, providing evidence that this complex is involved in synaptic vesicle docking and fusion at the presynaptic terminal.
 
Technical Info/Product Notes:Recommended by the Human Protein Atlas Organization for IHC (Ensembl No. ENSG00000180104).
 
VAM-SV021 WB
Western blot analysis of pig ESK-4 cell lysate, probed with Prod. No. ADI-VAM-SV021.
VAM-SV021 IHC
Immunohistochemistry analysis of human tonsil tissue stained with rSec6, mAb (9H5) at 10µg/ml.
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VAM-SV021 WB VAM-SV021 IHC

Product Literature References

Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct: M. Knepper, et al. ; Am. J. Physiol. Cell Physiol. 295, C661 (2008), Application(s): WB using rat tissue, Abstract;
Three-dimensional analysis of post-Golgi carrier exocytosis in epithelial cells: E. Rodriguez-Boulan, et al. ; Nat. Cell Biol. 5, 126 (2003), Application(s): ICC using canine samples, Abstract;
The brain exocyst complex interacts with RalA in a GTP-dependent manner: identification of a novel mammalian Sec3 gene and a second Sec15 gene: P.J. Robinson, et al. ; J. Biol. Chem. 276, 29792 (2001), Application(s): WB using rat samples, Abstract;

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