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NSF monoclonal antibody (9G7-3)

 
ADI-VAM-SV020-D 50 µg 194.00 USD
 
ADI-VAM-SV020-F 200 µg 370.00 USD
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Product Specification

Alternative Name:N-Ethylmaleimide-sensitive fusion protein
 
Clone:9G7-3
 
Host:Mouse
 
Isotype:IgG2b
 
Immunogen:Recombinant bovine NSF.
 
UniProt ID:P46459 (human)
 
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Dog, Hamster, Monkey, Porcine, Rabbit, Sheep
 
Applications:IHC (PS), WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~76kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:N-ethylmaleimide-sensitive factor (NSF) is involved in a variety of membrane fusion events and plays a prominent role in synaptic vesicle fusion. In order to interact with target membranes, NSF requires another set of soluble proteins called Soluble NSF attachment proteins (SNAPs). Together with synaptotagmin and alpha-SNAP, NSF modulates the interaction of SNAP25, VAMP, and syntaxin in an ATP-dependent manner to form a 20S complex involved in synaptic vesicle docking and fusion.
 
VAM-SV020 WB
Western blot analysis: Lane 1: MW marker, Lane 2: HeLa (Heat Shocked), Lane 3: Vero, Lane 4: CHO-K1, Lane 5: ESK-4.
VAM-SV020 IHC
Immunohistochemistry analysis of human testis tissue stained with NSF, mAb (9G7-3) at 20µg/ml.
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VAM-SV020 WB VAM-SV020 IHC

Product Literature References

A quantitative study on the expression of synapsin II and N-ethylmaleimide-sensitive fusion protein in schizophrenic patients: H. Nawa, et al. ; Neurosci. Lett. 305, 185 (2001), Application(s): WB using human samples, Abstract;
Three novel proteins of the syntaxin/SNAP-25 family: R.H. Scheller, et al. ; J. Biol. Chem. 273, 34171 (1998), Application(s): WB using monkey samples, Abstract;

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