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rSec8 monoclonal antibody (14G1)

 
ADI-VAM-SV016-D 50 µg 205.00 USD
 
ADI-VAM-SV016-F 200 µg 399.00 USD
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Product Specification

Alternative Name:Exocyst complex component 4
 
Clone:14G1
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant rat rSec8.
 
UniProt ID:Q62824
 
GenBank ID:U32498
 
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Dog, Fish, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep
 
Applications:ICC, IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Immunoprecipitation (12.5µg/ml)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~110kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:rSec6 and rSec8 are rat homologs of the yeast Sec6 and Sec8 proteins that are essential for protein transport between the Golgi apparatus and the plasma membrane. In yeast, Sec8 is part of a 19.5S complex that includes Sec6 and Sec15, that also associate with the ras-like GTP binding protein Sec4. An analogous 17S complex in rat brain was found to contain rSec6 and rSec8, along with eight proteins with a combined molecular weight of 743 kDa. The membrane-associated rSec6/8 complex co-immunoprecipitates with syntaxin, providing evidence that this complex is involved in synaptic vesicle docking and fusion at the presynaptic terminal.
 
VAM-SV016 IHC
Immunohistochemistry analysis of human brain, cerebellum tissue stained with rSec8, mAb (14G1) at 5µg/ml.
VAM-SV016 WB 01
Western blot analysis of rSec8: Lane 1: MWM, Lane 2: Brain (rat), (tissue extract) (Prod No. ADI-LYT-RB100), Lane 3: Brain (mouse), (tissue extract) (Prod No. ADI-LYT-MB100), Lane 4: MDBK Cell Lysate.
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VAM-SV016 IHC VAM-SV016 WB 01

Product Literature References

Arl13b and the exocyst interact synergistically in ciliogenesis: C. Seixas, et al.; Mol. Biol. Cell. 27, 308 (2016), Application(s): Immunoblotting of mouse kidney lysates, Abstract; Full Text
Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct: M. Knepper, et al. ; Am. J. Physiol. Cell Physiol. 295, C661 (2008), Application(s): WB using rat tissue, Abstract;
PALS1 regulates E-cadherin trafficking in mammalian epithelial cells: B. Margolis, et al. ; Mol. Biol. Cell 18, 874 (2007), Application(s): ICC, WB using canine tissue culture, Abstract;
Ral-GTPase influences the regulation of the readily releasable pool of synaptic vesicles: T.J. Turner, et al. ; Mol. Cell. Biol. 22, 1714 (2002), Application(s): WB using mouse samples, Abstract;

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Review for rSec8 monoclonal antibody (14G1)


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