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VAMP2 monoclonal antibody (SP10)

 
ADI-VAM-SV014-C 25 µl 165.00 USD
 
ADI-VAM-SV014-E 100 µl 281.00 USD
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Product Specification

Alternative Name:Synaptobrevin, Vesicle-associated membrane protein 2
 
Clone:SP10
 
Host:Mouse
 
Isotype:IgM
 
Immunogen:Synaptic vesicle-containing fractions immunoprecipitated from human brain homogenates using anti-human Synaptophysin monoclonal antibodies.
 
UniProt ID:P23763 (VAMP1), P63027
 
GenBank ID:M36200 (VAMP1), M36205 (VAMP2)
 
Species reactivity:Human, Mouse, Rat
Bovine, Hamster, Porcine, Xenopus
 
Applications:IHC, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (1:50, human neuroblastoma tissue)
Western Blot (1:4,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~18kDa by Western blot.
 
Purity Detail:Ammonium sulfate precipitation purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 0.1mM PMSF and 50% glycerol.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Vesicle-associated membrane protein (VAMP), also known as synaptobrevin, is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicles. Two VAMP homologs, VAMP1 and VAMP2, are differentially expressed in the nervous system. VAMP2 is found in neurons associated with autonomic, sensory and integrative functions, and in non-neuronal Langerhans islets and glomerular cells. VAMP is a component of a 20S complex implicated in vesicle docking and fusion, the formation of which involves the interaction of VAMP with syntaxin through an ATP-dependent reaction modulated by synaptotagmin, SNAP-25, alpha-SNAP, and NSF.
 
VAM-SV014 WB
Western blot analysis of mouse brain tissue extract (Prod. No. ADI-LYT-MB100) probed with VAMP2 (Synaptobrevin) mAb.
VAM-SV014 IHC1
Immunohistochemistry analysis of human neuroblastoma tissue immunohistochemically stained using VAMP mAb (SP10) at a dilution of 1:50.
VAM-SV014 IHC2
Immunohistochemistry analysis of Xenopus embryonic brain, stained with VAMP2, mAb (SP10).
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VAM-SV014 WB VAM-SV014 IHC1 VAM-SV014 IHC2

Product Literature References

Aquaporin 1 regulates GTP-induced rapid gating of water in secretory vesicles: B.P. Jena, et al. ; PNAS 99, 4720 (2002), Application(s): WB using rat samples, Abstract;
The 26-mer peptide released from SNAP-25 cleavage by botulinum neurotoxin E inhibits vesicle docking: M. Montal, et al. ; FEBS Lett. 435, 84 (1998), Application(s): WB using rat samples, Abstract;

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