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Synaptophysin monoclonal antibody (SP15)

 
ADI-VAM-SV011-D 50 µl 219.00 USD
 
ADI-VAM-SV011-F 200 µl 404.00 USD
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Product Specification

Alternative Name:SYP
 
Clone:SP15
 
Host:Mouse
 
Isotype:IgM
 
Immunogen:Synaptic vesicle-containing fractions immunoprecipitated from human brain homogenates using anti-human Synaptophysin mAb antibodies.
 
UniProt ID:P08247
 
Species reactivity:Human, Mouse, Rat
Bovine
 
Applications:IHC, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (1:50, human neuroblastoma tissue)
Western Blot (1:2,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~38kDa by Western blot.
 
Purity Detail:Ammonium sulfate precipitation purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Synaptophysin is an integral membrane protein involved in neurotransmitter exocytosis. This protein consists of four transmembrane domains, with its N- and C-terminus facing the cytoplasm. Studies have shown Synaptophysin to be a major cholesterol-binding protein in brain synaptic vesicles.
 
ADI-VAM-SV011 WB
Western blot analysis of Synaptophysin, mAb (SP15) (Prod. No. ADI-VAM-SV011): Lane 1: MW marker; Lane 2: Mouse brain extract (Prod. No. ADI-LYT-MB100); Lane 3: Rat brain extract (Prod. No. ADI-LYT-RB100)
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ADI-VAM-SV011 WB

Product Literature References

The development of mastication in rodents: From neurons to behaviors: J.E. Turman Jr., et al.; Arch. Oral Biol. 52, 313 (2007), Application(s): IHC using mouse tissue, Abstract;
Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex: P. Davies, et al. ; Brain Res. 609, 20 (1993), Application(s): WB using human samples, Abstract;

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