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SAP97 monoclonal antibody (RPI 197.4)

ADI-VAM-PS005-D 50 µg 188.00 USD
ADI-VAM-PS005-F 200 µg 397.00 USD
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Product Specification

Alternative Name:Synapse-associated protein 97, Disks large homolog 1
Clone:RPI 197.4
Immunogen:Recombinant rat SAP97 (N-terminal fragment).
UniProt ID:Q62696
Species reactivity:Human, Mouse, Rat
Applications:ICC, IHC, IP, WB
Electron microscopy
Recommended Dilutions/Conditions:Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects bands of ~97kDa and ~140kDa by Western blot.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Synapse-associated proteins SAP97 and SAP102 display extensive sequence homology to the PSD 95 family of proteins that facilitate ion channel clustering at synaptic terminals. In addition to the CNS, SAP97 is also detected at the basal lateral membrane between a variety of epithelial cells. SAP102 associates with NMDA receptors through the C-terminal NR2B subunit of the receptor. The N-terminal SAP97 PDZ domain mediates its interaction with the synaptic ras-GTPase activating protein SynGAP.
Western blot analysis: Lane 1: MW marker, Lane 2: Rat Brain Tissue Extract, Lane 3: Mouse Brain Tissue Extract.
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Product Literature References

Modulatory effect of acetyl-L-carnitine on amyloid precursor protein metabolism in hippocampal neurons: M. Di Luca, et al. ; Eur. J. Pharmacol. 597, 51 (2008), Application(s): WB using human cell lysates, Abstract;
A novel and conserved protein-protein interaction domain of mammalian Lin-2/CASK binds and recruits SAP97 to the lateral surface of epithelia: B. Margolis, et al. ; Mol. Cell. Biol. 22, 1778 (2002), Application(s): IP, ICC, WB using canine samples, Abstract;
Molecular mechanisms regulating the differential association of kainate receptor subunits with SAP90/PSD-95 and SAP97: J. Marshall, et al. ; J. Biol. Chem. 276, 16092 (2001), Application(s): WB using human & rat samples, Abstract;

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