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Bassoon monoclonal antibody (SAP7F407)

ADI-VAM-PS003-D 50 µg 198.00 USD
ADI-VAM-PS003-F 200 µg 418.00 USD
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Product Specification

Immunogen:Recombinant rat Bassoon.
UniProt ID:O88778
Gene/Protein Identifier:29999 (UniGene)
Source:Purified from ascites.
Species reactivity:Mouse, Rat
Applications:ICC, IF, IHC, IP, WB
Recommended Dilutions/Conditions:Immunocytochemistry (1:400)
Immunoprecipitation (2.5µg)
Western Blot (1:500 - 1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~400kDa by Western blot.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Bassoon is a 420 kDa protein that localizes at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors. These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release.
Western blot analysis of Bassoon: Lane 1: MW marker, Lane 2: Rat Brain Tissue Lysate.
Immunohistochemistry analysis of bassoon localization in the rat brain cerebellar molecular layer using Bassoon, mAb (SAP7F407).
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Product Literature References

Dual-color STED microscopy reveals a sandwich structure of Bassoon and Piccolo in active zones of adult and aged mice: H. Nishimune, et al.; Sci. Rep. 6, 27935 (2018), Abstract; Full Text
Structural insights into modulation and selectivity of transsynaptic neurexin-LRRTM interaction: A. Yamagata, et al.; Nat. Commun. 9, 3964 (2018), Abstract; Full Text
A single dose of docosahexaenoic acid increases the functional recovery promoted by rehabilitation following cervical spinal cord injury in the rat: Z.H. Liu, et al.; J. Neurotrauma 34, 1766 (2017), Abstract;
Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors: C. Puller, et al.; PLoS One 12, e0173455 (2017), Abstract; Full Text
Monoacylglycerol lipase inhibitor JZL184 prevents HIV-1 gp120-induced synapse loss by altering endocannabinoid signaling: X. Zhang, et al.; Neuropharmacology 128, 269 (2017), Abstract;
Rapid Sequential in Situ Multiplexing with DNA Exchange Imaging in Neuronal Cells and Tissues: Y. Wang, et al.; Nano Lett. 17, 6131 (2017), Abstract; Full Text
Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking: S.S. Wang, et al.; Neuron 91, 777 (2016), Application(s): Immunostaining, Immunoblotting, Abstract;
Grafted Human iPS Cell-Derived Oligodendrocyte Precursor Cells Contribute to Robust Remyelination of Demyelinated Axons after Spinal Cord Injury: S. Kawabata, et al. ; Stem Cell Reports 6, 1 (2016), Application(s): Immunohistochemistry, Abstract;
Impairment of Vision in a Mouse Model of Usher Syndrome Type III: G. Tian, et al.; Invest. Ophthalmol. Vis. Sci. 57, 866 (2016), Application(s): Immunofluorescence staining, Abstract; Full Text
Periodic F-actin structures shape the neck of dendritic spines: J. Bär, et al.; Sci. Rep. 6, 37136 (2016), Abstract; Full Text
Dopaminergic control of autophagic-lysosomal function implicates Lmx1b in Parkinson's disease: A. Laguna, et al.; Nat. Neurosci. 18, 826 (2015), Application(s): Immunohistochemistry, Abstract;
GSK3β Inhibition Promotes Synaptogenesis in Drosophila and Mammalian Neurons: G. Cuesto, et al.; PLoS One 10, e0118475 (2015), Application(s): Cell Culture, Abstract; Full Text
Mechanisms of splicing-dependent trans-synaptic adhesion by PTPδ-IL1RAPL1/IL-1RAcP for synaptic differentiation: A. Yamagata, et al.; Nat. Commun. 6, 6926 (2015), Application(s): Immunostaining, Abstract; Full Text
The phospholipid-binding protein SESTD1 negatively regulates dendritic spine density by interfering with Rac1-Trio8 signaling pathway: C. Lee, et al.; Sci. Rep. 5, 13250 (2015), Application(s): Immunofluorescence, Abstract;
TIMP-1 affects the spatial distribution of dendritic processes of second-order neurons in a rat model of Retinitis Pigmentosa: J. Shin, et al. ; Exp. Eye Res. 140, 41 (2015), Application(s): Immunohistochemistry, Abstract;
Acute mechanisms underlying antibody effects in anti–N‐methyl‐D‐aspartate receptor encephalitis: E.H. Moscato, et al.; Ann. Neurol. 76, 108 (2014), Application(s): Immunocytochemistry using rat hippocampal neurons, Abstract; Full Text
ArfGAP3 is a component of the photoreceptor synaptic ribbon complex and forms an NAD(H)-regulated, redox-sensitive complex with RIBEYE that is important for endocytosis: M. Dembla, et al.; J. Neurosci. 34, 5245 (2014), Application(s): Immunohistochemistry using murine retina sections, Abstract; Full Text
LKB1 and AMPK regulate synaptic remodeling in old age: M.A. Samuel, et al.; Nat. Neurosci. 17, 1190 (2014), Application(s): Immunohistochemistry and Western blot using murine retina tissue samples, Abstract; Full Text
Co-regulation of intragenic microRNA miR-153 and its host gene Ia-2β: identification of miR-153 target genes with functions related to IA-2β in pancreas and brain: W. Mandemakers, et al.; Diabetologia 56, 1547 (2013), Application(s): WB analysis of endogenous bassoon in SNAP25, SNCA, parkin and beta actin from SH-SY5Y cells, Abstract; Full Text
N-Cadherin Relocalizes from the Periphery to the Center of the Synapse after Transient Synaptic Stimulation in Hippocampal Neurons: P.T. Yam, et al.; PLoS One 8, e79679 (2013), Application(s): Immunostaining Bassoon staining at synapses in paraformaldehyde fixed neurons from Sprague Dawley rats, Abstract; Full Text
Protein composition of immunoprecipitated synaptic ribbons: A. Kantardzhieva, et al. ; J. Proteome Res. 11, 1163 (2012), Application(s): Immunolabeling of mouse retinas, Abstract; Full Text
Calcium Channels Link the Muscle-Derived Synapse Organizer Laminin β2 to Bassoon and CAST/Erc2 to Organize Presynaptic Active Zones: J. Chen, et al.; J. Neurosci. 21, 512 (2011), Application(s): Visualized by fluorescent immunohistochemistry using an anti-Bassoon antibody, Abstract; Full Text
γ-Protocadherins regulate neuronal survival but are dispensable for circuit formation in retina: J. Sanes, et al. ; Development 135, 4141 (2008), Application(s): IHC using mouse tissue, Abstract;
Mechanisms of prolonged presynaptic Ca(2+) signaling and glutamate release induced by TRPV1 activation in rat sensory neurons: Y. Usachev, et al. ; J. Neurosci. 28, 5295 (2008), Application(s): ICC, IF using rat tissue culture, Abstract;
Polarized Targeting of Neurexins to Synapses Is Regulated by their C-Terminal Sequences: M. Missler, et al. ; J. Neurosci. 28, 12969 (2008), Application(s): IHC using mouse tissue culture, Abstract;
Retinal cells integrate into the outer nuclear layer and differentiate into mature photoreceptors after subretinal transplantation into adult mice: M. Ader, et al. ; Exp. Eye Res. 86, 691 (2008), Application(s): IHC, IF using mouse tissue, Abstract;
Extensive neuronal differentiation of human neural stem cell grafts in adult rat spinal cord: V. Koliatsos, et al. ; PLoS Med. 4, 318 (2007), Application(s): IHC using rat tissue, Abstract;
Neuronal differentiation and synaptogenesis in retinoblastoma: D.A. Johnson, et al.; Cancer Res. 67, 2701 (2007), Application(s): IHC, IF using mouse tissue & tissue culture, Abstract;
Ultrastructural localization of active zone and synaptic vesicle proteins in a preassembled multi-vesicle transport aggregate.: J.H.Tao-Cheng, et al.; Neuroscience 150, 575 (2007), Application(s): Immunogold labeling of Bassoon in dissociated hippocampal neuronal cultures, Abstract; Full Text
Phosphorylation of the AMPA receptor GluR1 subunit is required for synaptic plasticity and retention of spatial memory: R.L. Huganir, et al. ; Cell 112, 631 (2003), Application(s): ICC using mouse samples, Abstract;
Rapid and differential regulation of AMPA and kainate receptors at hippocampal mossy fibre synapses by PICK1 and GRIP: J.M. Henley, et al. ; Neuron 37, 625 (2003), Application(s): WB using rat samples, Abstract;
CAST: a novel protein of the cytomatrix at the active zone of synapses that forms a ternary complex with RIM1 and munc13-1: Y. Takai, et al. ; J. Cell Biol. 158, 577 (2002), Application(s): IP, WB, IHC using rat & mouse samples, Abstract;
The presynaptic particle web: ultrastructure, composition, dissolution, and reconstitution: D.R. Colman, et al. ; Neuron 32, 63 (2001), Application(s): WB using rat samples, Abstract;

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