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Synaptotagmin monoclonal antibody (ASV48)

 
ADI-SYA-148-D 50 µg 194.00 USD
 
ADI-SYA-148-F 200 µg 399.00 USD
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Product Specification

Alternative Name:SYT
 
Clone:ASV48
 
Host:Mouse
 
Isotype:IgG2b
 
Immunogen:Rat brain synaptic junction protein complexes.
 
UniProt ID:P21707
 
GenBank ID:X52772
 
Species reactivity:Mouse, Rat
Monkey, Shark
 
Applications:ICC, IHC, IP, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:500)
Western Blot (0.1µg/ml, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~65kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Synaptotagmins (Syt) are membrane-trafficking proteins characterized by an N-terminal transmembrane region, a variable linker, and two C-terminal C2-domains, C2A and C2B. At least twelve Synaptotagamins are expressed in vertebrates and can be found in both vesicular and plasma membranes.
 
SYA-148 WB
Western blot analysis of rat brain tissue extract, probed with Synaptotagmin, mAb (ASV48).
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SYA-148 WB

Product Literature References

Nerve growth factor-dependent sorting of synaptotagmin IV protein to mature dense-core vesicles that undergo calcium-dependent exocytosis in PC12 cells: A. Yamamoto, et al. ; J. Biol. Chem. 278, 3220 (2003), Application(s): ICC using rat samples, Abstract;
Synaptotagmin IX regulates Ca2+-dependent secretion in PC12 cells: K. Mikoshiba, et al. ; J. Biol. Chem. 277, 4601 (2002), Application(s): ICC using rat samples, Abstract;
Synaptotagmin IV is present at the Golgi and distal parts of neurites: K. Mikoshiba, et al. ; J. Neurochem. 74, 518 (2000), Application(s): WB using mouse samples, Abstract;
Synaptotagmin IV is present at the Golgi and distal parts of neurites: K. Mikoshiba, et al. ; J. Neurochem. 74, 518 (2000), Application(s): ICC, WB using rat samples, Abstract;
Comparison of immunolocalization patterns for the synaptic vesicle proteins p65 and synapsin I in macaque monkey retina: A.E. Hendrickson, et al. ; Synapse 14, 268 (1993), Application(s): ICC using monkey samples, Abstract;
Identification of a synaptic vesicle-specific membrane protein with a wide distribution in neuronal and neurosecterory tissue: W.D. Matthew, et al.; J. Cell Biol. 91, 257 (1981), Application(s): WB, IP using fish (shark) & rat samples, Abstract;

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