Product Details
Alternative Name: | ESR |
|
Clone: | h-151 |
|
Host: | Mouse |
|
Isotype: | IgG1 |
|
Immunogen: | Synthetic peptide corresponding to the sequence of human estrogen receptor, conjugated to KLH. |
|
UniProt ID: | P03372 |
|
GenBank ID: | M12674 |
|
Source: | Purified from mouse ascites. |
|
Species reactivity: | Human, Rat
|
|
Applications: | ICC, IP, WB
|
|
Recommended Dilutions/Conditions: | Immunoprecipitation (15µg/ml) Western Blot (1:250) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
|
Application Notes: | Detects a band of ~67kDa by Western blot. |
|
Purity Detail: | Protein G affinity purified. |
|
Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
|
Handling: | Avoid freeze/thaw cycles. |
|
Shipping: | Blue Ice |
|
Long Term Storage: | -20°C |
|
Scientific Background: | The Estrogen receptors (ER), mainly ERalpha and ERbeta, are members of the nuclear steroid hormone receptor family and are composed of three domains, an N-terminal domain required for maximal transactivation function (TAF), a DNA binding domain, and a steroid binding domain. Estrogen has pleiotropic effects on the growth, development, and maintenance of diverse tissues. |
|
Regulatory Status: | RUO - Research Use Only |
|
Human Estrogen Receptor domains.
Western blot analysis of ESR1: Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa)
Please mouse over
Product Literature References
Reassessment of estrogen receptor expression in human breast cancer cell lines: C.H. Ford, et al.; Anticancer Res.
31, 521 (2011),
Application(s): Flow cytometry and immunofluorescence,
Abstract;
Serum-dependent effects of tamoxifen and cannabinoids upon C6 glioma cell viability: C.J. Fowler, et al. ; Biochem. Pharmacol.
60, 1807 (2000),
Application(s): WB using rat samples,
Abstract;
Rapid actions of estrogens in GH3/B6 pituitary tumor cells via a plasma membrane version of estrogen receptor-alpha: B. Gametchu, et al. ; Steroids
64, 13 (1999),
Application(s): ICC using human & rat samples,
Abstract;
An N-terminal inhibitory function, IF, suppresses transcription by the A-isoform but not the B-isoform of human progesterone receptors: K.B. Horwitz, et al. ; J. Biol. Chem.
273, 5455 (1998),
Application(s): WB using human samples,
Abstract;
AIB1, a steroid receptor coactivator amplified in breast cancer and ovarian cancer: P.S. Meltzer, et al. ; Science
277, 965 (1997),
Application(s): WB using human samples,
Abstract;
Related Products