Western Blot (1:10,000, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Application Notes:
Detects a band of ~60kDa by Western blot.
Purity Detail:
Protein G affinity purified.
Formulation:
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Handling:
Avoid freeze/thaw cycles.
Shipping:
Blue Ice
Long Term Storage:
-20°C
Scientific Background:
GroEL and GroES are E. coli chaperonins, which are homologs of mitochondrial chaperonins Hsp60 and Hsp10. GroEL is a double toriodal assembly of 14 identical subunits which form two heptameric rings stacked back-to-back, with a cavity at each end. GroEL and its co-chaperonin GroES facilitate protein folding with an ATP-dependent mechanism.
Regulatory Status:
RUO - Research Use Only
Western blot analysis of GroEL, mAb (9A1/2) (Prod. No. ADI-SPS-870): Lane 1: GroEL ( E. coli ) (rec) (Prod. No. ADI-SPP-610) Protein, Lane 2: HSP60 (Human) (rec) (negative control) (Prod. No. ADI-SPP-540), Lane 3: E. coli Lysate, Lane 4: HeLa Lysate, heat shocked (negative control) (Prod. No. ADI-LYC-HL101)
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Product Literature References
Unstable Mechanisms of Resistance to Inhibitors of Escherichia coli Lipoprotein Signal Peptidase: H. Pantua, et al.; Mbio 11, e02018-20 (2020), Abstract; Full Text
An NAD+ Phosphorylase Toxin Triggers Mycobacterium tuberculosis Cell Death: D.M. Freire, et al.; Mol. Cell 73, 1282 (2019), Abstract;
Defects in the mitochondrial-tRNA modification enzymes MTO1 and GTPBP3 promote different metabolic reprogramming through a HIF-PPARγ-UCP2-AMPK axis: R. Boutoual, et al.; Sci. Rep. 8, 1163 (2018), Application(s): Western-blot, Abstract; Full Text
Bacterial Heat Shock Protein GroEL (Hsp64) Exerts Immunoregulatory Effects on T Cells by Utilizing Apoptosis: A.M. Nalbant, et al.; PLoS One 11, e0164380 (2016), Application(s): Preparation of the GroEL antigen, Abstract; Full Text
Streptomycin Induced Stress Response in Salmonella enterica Serovar Typhimurium Shows Distinct Colony Scatter Signature: A.K. Singh, et al.; PLoS One 10, e0135035 (2015), Application(s): Immunoassays, Abstract; Full Text
The cost of antibiotic resistance depends on evolutionary history in Escherichia coli: D.C. Angst, et al.; BMC Evol. Biol. 13, 163 (2013), Application(s): Western-blot, Abstract; Full Text
Antisense downregulation of sigma 32 as a transient metabolic controller in Escherichia coli: effects on yield of active organophosphorus hydrolase: W.E. Bentley, et al. ; Appl. Environ. Microbiol. 66, 4366 (2000), Application(s): WB using bacteria (E. coli) samples, Abstract;
Protein oxidation in response to increased transcriptional or translational errors: T. Nystrom, et al. ; PNAS 97, 5746 (2000), Application(s): WB using bacteria (E. coli) samples, Abstract;
