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Grp75/Mortalin monoclonal antibody (30A5)

 
ADI-SPS-825-D 50 µl 281.00 USD
 
ADI-SPS-825-F 200 µl 486.00 USD
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Product Specification

Alternative Name:MtHsp70, Hsp70 (mitochondrial), HspA9
 
Clone:30A5
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Native human Grp75 (Mortalin).
 
UniProt ID:P38646
 
GenBank ID:L15189
 
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Dog, Drosophila, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus
 
Applications:IHC, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (1:50)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~75kDa by Western blot.
 
Purity Detail:Ammonium sulfate precipitation purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Grp75 (Glucose regulated 75 kDa protein) is a member of the Hsp70 family. It is localized in the mitochondrial matrix, where, in concert with Hsp60, is thought to participate in the re-folding of proteins translocated into this organelle. Like its E. coli homolog DnaK, Grp75 possesses a cation-dependent ATPase activity thought to be central to its function as a chaperone.
 
SPS-825 IHC
Immunohistochemistry analysis of human breast cancer tissue immunohistochemically stained using Grp75 mAb (30A5).
ADI-SPS-825 WB
Western blot analysis of Grp75/Mortalin, mAb (30A5) (Prod. no. ADI-SPS-825): Lane 1: MW marker; Lane 2: HeLa (heat shocked); Lane 3: L-929 (heat shocked); Lane 4: CHO -K1 (heat shocked); Lane 5: GPC-16 (heat shocked); Lane 6: PC-12 (heat shocked).
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SPS-825 IHC ADI-SPS-825 WB

Product Literature References

Toxic Memory' via chaperone modification is a potential mechanism for rapid Mallory-Denk body reinduction: M. Omary, et al. ; Hepatology 48, 931 (2008), Application(s): WB using mouse tissue, Abstract;

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