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HSP90α polyclonal antibody

 
ADI-SPS-771-D 50 µg 151.00 USD
 
ADI-SPS-771-F 200 µg 334.00 USD
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Product Specification

Alternative Name:HSP86, Heat shock protein 90α
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to a portion of mouse Hsp86.
 
UniProt ID:P07901
 
GenBank ID:X16857
 
Species reactivity:Human, Mouse, Rat
Beluga, Bovine, Dog, Fish, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus
 
Applications:IHC, WB
Cell migration, Inhibition assay
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~90kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS containing 0.09% sodium azide and 50% glycerol.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress, and includes cytoplasmic (Hsp90-alpha/beta), ER (grp94), and mitochondrial (TRAP1) localized members. Structurally, Hsp90 is characterized by an N-terminal ATP-binding domain, a medial substrate-binding domain, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones (e.g. Hsp40, Hsp70, Hop, p23) to stabilize a multitude of client protein substrates, including steroid hormone receptors, protein kinases, and transcription factors. The essential binding and hydrolysis of ATP by Hsp90 is inhibited by ansamycin drugs (e.g. geldanamycin, 17-AAG) which occupy the N-terminal Hsp90 nucleotide-binding pocket. Many Hsp90 client proteins such as erbB2/Her-2, c-raf, bcr-abl, p53, and hTERT, are members of well characterized oncogenic pathways, making Hsp90 inhibitors useful anticancer agents.
 
ADI-SPS-771 WB
Western blot analysis of HSP90α, pAb (Prod. No. ADI-SPS-771): Lane 1: MW Marker, Lane 2: HeLa Cell Lysate (heat shocked) (Prod. No. ADI-LYC-HL101), Lane 3: PC-12 Cell Lysate (heat shocked) (Prod. No. ADI-LYC-PC101), Lane 4: 3T3 Cell Lysate (heat shocked) (Prod. No. ADI-LYC-3T101), Lane 5: CHO-K1 Cell Lysate (heat shocked), Lane 6: MDBK Cell Lysate (heat shocked), Lane 7: MDCK Cell Lysate (heat shocked), Lane 8: Vero Cell Lysate (heat shocked), Lane 9: RK-13 Cell Lysate (heat shocked)
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ADI-SPS-771 WB

Product Literature References

Alpha B-Crystallin Protects Rat Articular Chondrocytes against Casein Kinase II Inhibition-Induced Apoptosis: S.W. Lee, et al.; PLoS One 11, e0166450 (2016), Abstract; Full Text
Oxidation and interaction of DJ-1 with 20S proteasome in the erythrocytes of early stage Parkinson’s disease patients: Y. Saito, et al.; Sci. Rep. 6, 30793 (2016), Application(s): SDS-PAGE and 2D-PAGE for western blotting, Abstract; Full Text
Linking HSP90 target occupancy to HSP70 induction and efficacy in mouse brain: K. Thirstrup, et al.; Pharmacol. Res. 104, 197 (2015), Application(s): Immunoblot analysis using mouse cell and tissue homogenates, Abstract;
Extracellular heat shock protein-90α: linking hypoxia to skin cell motility and wound healing: W. Li, et al. ; EMBO J. 26, 1221 (2007), Application(s): IA, Cell Migration using human culture supernatants, Abstract;
Male fetal germ cells are targets for androgens that physiologically inhibit their proliferation: C. Racine, et al. ; PNAS 104, 3615 (2007), Application(s): IHC using mouse tissue, Abstract;
The inhibitory action of sodium arsenite on lipopolysaccharide-induced nitric oxide production in RAW 267.4 macrophage cells: a role of Raf-1 in lipopolysaccharide signaling: T. Yokochi, et al. ; J. Immunol. 166, 2011 (2001), Application(s): WB using mouse samples, Abstract;

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