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ERp72 polyclonal antibody

 
ADI-SPS-720-D 50 µg 161.00 USD
 
ADI-SPS-720-F 200 µg 356.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:PDIA4, ERP70, protein disulfide isomerase
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 623-638 of mouse ERp72.
 
UniProt ID:P08003
 
Species reactivity:Human, Mouse, Rat
Bovine, Dog, Guinea pig, Hamster, Monkey, Rabbit
 
Applications:IF, IHC (PS), WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~72kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Endoplasmic reticulum protein (ERp) 72, a 72 kDa protein localized to the endoplasmic reticulum, is a member of the Protein Disulfide Isomerase (PDI) family of proteins. Together with other ER resident proteins such as BiP, GRP94, and PDI, they serve as the molecular chaperones for proper folding of newly translocated and glycolsylated proteins such as Thyroglobulin (Tg) and Human chorionic gonadotropin (hCG)-beta.
 
Technical Info/Product Notes:Recommended by the Human Protein Atlas Organization for IHC (Ensembl No. ENSG00000155660).
 
SPS-720 WB
Western blot analysis of ERp72: Lane 1: MW Marker, Lane 2: Rat Liver Microsome Extract, Lane 3: Mouse Liver Microsome Extract, Lane 4: Human Liver Microsome Extract.
ADI-SPS-720 IHC1
IHC Analysis: Human brain, cerebellum tissue stained with ERp72, pAb at 10µg/ml.
ADI-SPS-720 IHC2
IHC Analysis: Human placenta tissue stained with ERp72, pAb at 10µg/ml.
ADI-SPS-720 IHC3
IHC Analysis: Human prostate tissue stained with ERp72, pAb at 10µg/ml.
ADI-SPS-720 IHC4
IHC Analysis: Human testis tissue stained with ERp72, pAb at 10µg/ml.
Please mouse over
SPS-720 WB ADI-SPS-720 IHC1 ADI-SPS-720 IHC2 ADI-SPS-720 IHC3 ADI-SPS-720 IHC4

Product Literature References

Redox sensitivities of global cellular cysteine residues under reductive and oxidative stress: K. Araki, et al.; J. Proteome Res. 15, 2548 (2016), Application(s): Western blot, Abstract;
The C-terminal CGHC motif of protein disulfide isomerase supports thrombosis: J. Zhou, et al.; J. Clin. Invest. 125, 4391 (2015), Application(s): Western blot, Abstract; Full Text
∆F508 CFTR interactome remodelling promotes rescue of cystic fibrosis: S. Pankow, et al.; Nature. 528, 510 (2015), Application(s): Immunofluorescence, Abstract; Full Text
Glucose regulation of load-induced mTOR signaling and ER stress in mammalian heart: S. Sen, et al.; J. Am. Heart. Assoc. 2, e004796 (2013), Application(s): Western blot, Abstract; Full Text
Synergistic cooperation of PDI family members in peroxiredoxin 4-driven oxidative protein folding: Y. Sato, et al.; Sci. Rep. 3, 2456 (2013), Application(s): Western blot, Abstract; Full Text
Action of protein disulfide isomerase on proinsulin exit from endoplasmic reticulum of pancreatic β-cells: G. Raipal, et al.; J. Biol. Chem. 287, 43 (2012), Application(s): Western blot, Abstract; Full Text
The VP1 subunit of JC polyomavirus recapitulates early events in viral trafficking and is a novel tool to study polyomavirus entry: C.D. Nelson, et al.; Virology. 428, 30 (2012), Application(s): Western blot, Abstract; Full Text
A possible biochemical link between NADPH oxidase (Nox) 1redox-signalling and ERp72: T. Kamata, et al. ; Biochem. J. 416, 55 (2008), Application(s): WB, IF using human samples, Abstract;

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