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HSF1 (phosphorylated) (human), (recombinant) (His-tag)

 
ADI-SPP-902-D 50 µg 261.00 USD
 
ADI-SPP-902-F 200 µg 509.00 USD
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Product Specification

Alternative Name:HSTF1, Heat shock factor protein 1
 
Recommended Dilutions/Conditions:Western Blot (10ng, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
MW:~60kDa (predicted), ~70kDa (observed due to His-tag and hyperphosphorylation)
 
Source:Produced in E. coli. Human phosphorylated HSF1 is fused at the N-terminus to a His-tag.
 
UniProt ID:Q00613
 
Formulation:Liquid. In 1x TRIS buffered saline containing 5% glycerol.
 
Purity:≥90% (~60% full-length with trace upstream kinase)
 
Purity Detail:Purified by multi-step chromatography.
 
Applications:WB
 
Application Notes:Western blot control.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. The expression of heat shock proteins is mediated by the binding of a heat shock transcriptional factor (HSF) to a heat shock response element (HSE) which is located upstream of heat shock protein genes. Two heat shock transcriptional factors, termed HSF1 and HSF2, have been identified in mice and humans. In unstressed cells, HSF1 is a non-DNA binding monomeric protein present in the cytosol and nuclear compartments. However, in response to heat and heavy metals, HSF1 rapidly converts to a transcriptionally active form - a process requiring oligomerization to a trimer, serine phosphorylation, and translocation to the nucleus. HSF2, on the other hand, is not activated by heat, but is involved in regulating heat shock gene transcription during cell differentiation and development.
 
SPP-902 SDS-PAGE
SDS-PAGE analysis: Lane 1: MWM, Lane 2: 2µg HSF1, Lane 3: 2µg HSF1 (phospho)
SPP-902 WB1
Western Blot analysis: Lane 1: MW marker, Lane 2: HSF1, Lane 3: HSF1 (phospho) probed with HSF1 pAb
SPP-902 WB2
Western Blot analysis: Lane 1: MWM, Lane 2: HSF1, Lane 3: HSF1 (phospho) probed with HSF1 (pSer326) pAb
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SPP-902 SDS-PAGE SPP-902 WB1 SPP-902 WB2

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Heat Shock Proteins & the Cellular Stress Response Catalog
Heat Shock Proteins & the Cellular Stress Response Catalog
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