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PDI (human), (recombinant) (His-tag)

 
ADI-SPP-891-D 50 µg 173.00 USD
 
ADI-SPP-891-F 200 µg 458.00 USD
 
ADI-SPP-891-J 1 mg 1,594.00 USD
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Product Specification

Alternative Name:P4HB, protein disulfide isomerase
 
MW:~58kDa
 
Source:Produced in E. coli. Human PDI is fused at the N-terminus to a His-tag.
 
UniProt ID:P07237
 
Formulation:Liquid. In 50mM TRIS, pH 7.5, containing 150mM sodium chloride, 1mM DTT, 1mM EDTA, and 5% glycerol.
 
Purity:≥95% (SDS-PAGE; Western blot)
 
Purity Detail:Purified by multi-step chromatography.
 
Activity:Positive for disulfide isomerase activity, measured by promotion of insulin aggregation in the presence of DTT.
 
Specific Activity:>200 U/mg. 1 U increases the A650 of a 1mg/ml insulin solution by .01 OD/min at 25°C in the presence of DTT.
 
Applications:WB
Activity assay
 
Application Notes:Western blot control.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:The mammalian protein disulfide-isomerase (PDI) family encompasses several highly divergent proteins involved in the processing and maturation of secretory proteins in the ER by catalyzing the rearrangement of disulfide bonds. PDI, an abundant protein of the ER (>400uM), contains a carboxy-terminal retention signal sequence, KDEL, similar to that of BiP and Grp94. The PDI proteins are characterized by the presence of one or more domains of ~95-110 amino acids related to the cytoplasmic protein thioredoxin. All but the PDI-D subfamily are composed entirely of repeats of such domains, with at least one domain containing – and one domain lacking – a redox-active Cys-X-X-Cys tetrapeptide. In addition to roles as redox catalysts and isomerases, PDI proteins perform such functions as peptide binding and cell adhesion, and may conduct chaperone activities1. Platelet surface thiols and disulfides play an important role in platelet responses. Catalytically active PDI resides on platelet surfaces where it mediates platelet aggregation and secretion by reducing disulfide bonds, thus exposing fibrinogen receptors in platelets.
 
SPP-891 SDS-PAGE
SDS-PAGE analysis: Lane 1: MW marker, Lane 2: 0.5µg, Lane 3: 1µg, Lane 4: 2µg, Lane 5: 5µg PDI (human), (recombinant) (His-tag).
SPP-891 WB
Western Blot analysis: Lane 1: MWM, Lane 2: 50 ng PDI (human), (recombinant) (His-tag), probed with Prod. No. ADI-SPA-891.
SPP-891 AA
Enzymatic Activity: 1mg/ml insulin incubated with PDI in the presence of DTT – turbidity measured in 96-well microplate reader at 2 min intervals.
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SPP-891 SDS-PAGE SPP-891 WB SPP-891 AA

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