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TRAP1 (human), (recombinant) (His-tag)

 
ADI-SPP-848-050 50 µg 208.00 USD
 
ADI-SPP-848-200 200 µg 494.00 USD
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Product Specification

Alternative Name:HSP75, Mitochondrial HSP90, MtHSP90
 
Recommended Dilutions/Conditions:Western Blot (100ng, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
MW:~72kDa
 
Source:Produced in E. coli. Human TRAP1 (aa 60-704) is fused at the N-terminus to a His-tag.
 
UniProt ID:Q12931
 
Formulation:Liquid. In 50mM TRIS, pH 7.5, containing 150mM sodium chloride, 1mM DTT, and 1mM EDTA.
 
Purity:≥95% (SDS-PAGE; Western blot)
 
Purity Detail:Purified by multi-step chromatography.
 
Endotoxin Content:<50EU/mg purified protein (LAL test)
 
Applications:WB
 
Application Notes:Western blot control.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress. It includes cytoplasmic (Hsp90-alpha/beta), ER (Grp94) and mitochondrial (TRAP1) localized members. Hsp90 is structurally composed of an N-terminal ATP binding domain, a medial substrate-binding region, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones to stabilize a multitude of client proteins substrates. TRAP1 (aka Hsp75) was originally identified as a chaperone binding partner for retinoblastoma protein. TRAP1 was later found by immunofluorescence to be localized to the mitochondria. TRAP1 is significantly more active as an ATPase due in part to the lack of regulatory elements in the C-terminus, but retains the ability to dimerize. However, it does not form stable complexes with typical cochaperones of Hsp90 like p23 and HOP4. TRAP1 has been identified as a protective element for cell survival, and has been suggested as a target for anti-cancer therapeutics.
 
SPP-848 SDS-PAGE
SDS-PAGE analysis: Lane 1: MW marker, Lane 2: 1µg, Lane 3: 2µg, Lane 4: 5µg TRAP1 protein
SPP-848 WB
Western Blot analysis: Lane 1: MWM, Lane 2: TRAP1, probed with TRAP1 mAb.
SPP-848 ATPase
ATPase Assay: ATP hydrolysis after 1 hour at 37oC detected by malachite green. Quantities determined by comparison to potassium phosphate standard. Approximate activity is 1.2 nmol PO4/nmol TRAP1/min.
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SPP-848 SDS-PAGE SPP-848 WB SPP-848 ATPase

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Heat Shock Proteins & the Cellular Stress Response Catalog
Heat Shock Proteins & the Cellular Stress Response Catalog
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