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TRAP1 (human), (recombinant) (His-tag)

 
ADI-SPP-848-050 50 µg 218.00 USD
 
ADI-SPP-848-200 200 µg 519.00 USD
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Product Specification

Alternative Name:HSP75, Mitochondrial HSP90, MtHSP90
 
Recommended Dilutions/Conditions:Western Blot (100ng, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
MW:~72kDa
 
Source:Produced in E. coli. Human TRAP1 (aa 60-704) is fused at the N-terminus to a His-tag.
 
UniProt ID:Q12931
 
Formulation:Liquid. In 50mM TRIS, pH 7.5, containing 150mM sodium chloride, 1mM DTT, and 1mM EDTA.
 
Purity:≥95% (SDS-PAGE; Western blot)
 
Purity Detail:Purified by multi-step chromatography.
 
Endotoxin Content:<50EU/mg purified protein (LAL test)
 
Applications:WB
 
Application Notes:Western blot control.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress. It includes cytoplasmic (Hsp90-alpha/beta), ER (Grp94) and mitochondrial (TRAP1) localized members. Hsp90 is structurally composed of an N-terminal ATP binding domain, a medial substrate-binding region, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones to stabilize a multitude of client proteins substrates. TRAP1 (aka Hsp75) was originally identified as a chaperone binding partner for retinoblastoma protein. TRAP1 was later found by immunofluorescence to be localized to the mitochondria. TRAP1 is significantly more active as an ATPase due in part to the lack of regulatory elements in the C-terminus, but retains the ability to dimerize. However, it does not form stable complexes with typical cochaperones of Hsp90 like p23 and HOP4. TRAP1 has been identified as a protective element for cell survival, and has been suggested as a target for anti-cancer therapeutics.
 
TRAP1 (human), (recombinant) (His-tag) image
ATPase Assay: ATP hydrolysis after 1 hour at 37oC detected by malachite green. Quantities determined by comparison to potassium phosphate standard. Approximate activity is 1.2 nmol PO4/nmol TRAP1/min.
TRAP1 (human), (recombinant) (His-tag) Western blot
Western Blot analysis: Lane 1: MWM, Lane 2: TRAP1, probed with TRAP1 mAb.
TRAP1 (human), (recombinant) (His-tag) SDS-PAGE
SDS-PAGE analysis: Lane 1: MW marker, Lane 2: 1µg, Lane 3: 2µg, Lane 4: 5µg TRAP1 protein
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TRAP1 (human), (recombinant) (His-tag) image TRAP1 (human), (recombinant) (His-tag) Western blot TRAP1 (human), (recombinant) (His-tag) SDS-PAGE

Product Literature References

S-nitrosylation of the Mitochondrial Chaperone TRAP1 Sensitizes Hepatocellular Carcinoma Cells to Inhibitors of Succinate Dehydrogenase: S. Rizza, et al.; Cancer Res. 76, 4170 (2016), Abstract;
Structure-activity relationship in a purine-scaffold compound series with selectivity for the endoplasmic reticulum Hsp90 paralog Grp94: H.J. Patel, et al.; J. Med. Chem. 58, 3922 (2015), Abstract; Full Text
Experimental and structural testing module to analyze paralogue-specificity and affinity in the Hsp90 inhibitors series: T. Taldone, et al.; J. Med. Chem. 56, 6803 (2013), Abstract; Full Text
Paralog-selective Hsp90 inhibitors define tumor-specific regulation of HER2: P.D. Patel, et al.; Nat. Chem. Biol. 9, 677 (2013), Abstract; Full Text

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