United States
PERK (CT domain) (human), (recombinant) (His-tag)
| ADI-SPP-768-0050 | 50 µg | 180.00 USD |  |
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| ADI-SPP-768-0200 | 200 µg | 430.00 USD | |
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| Bulk Request | |||||
Recombinant human PERK, residues 536-1116 (accession No. NP_004827).
Product Specification
| Alternative Name: | EIF2AK3, Pek |
| MW: | ~69kDa |
| Source: | Produced in E. coli. |
| UniProt ID: | Q9NZJ5 |
| Formulation: | Liquid. In 50mM TRIS, pH 7.5, containing 100mM sodium chloride, 1mM dithiothreitol, and 20% glycerol. |
| Purity: | ≥95% (SDS-PAGE) |
| Purity Detail: | Purified by multi-step chromatography. |
| Application: | Western Blot (control) Optimal conditions must be determined individually for each application. |
| Short Term Storage: | -80°C |
| Long Term Storage: | -80°C |
| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C |
| Miscellaneous/General: | The endoplasmic reticulum (ER)-resident protein kinase PERK (also known as Pek, or Eukaryotic initiation factor 2 α-subunit kinase 3 (EIF2AK3)) attenuates protein synthesis in response to ER stress through the phosphorylation of translation initiation factor eIF2α on serine 51. A Type I membrane protein located in the ER, PERK is a critical regulator of the unfolded protein response (UPR). Defects in PERK are the cause of Wolcott-Rallison syndrome (WRS). |
Western Blot analysis of PERK protein (Prod. No. ADI-SPP-768) probed with anti-6x His tag antibody. Lane 1: MW marker, Lane2: 50 ng, Lane 3: 100 ng.
Western Blot analysis of PERK protein (Prod. No. ADI-SPP-768) probed with anti-PERK antibody. Lane 1: MW marker, Lane2: 50 ng, Lane 3: 100 ng.
SDS-PAGE Analysis: Lane 1: MW marker; Lane 2: 1.0 μg of purified PERK protein (Prod. No. ADI-SPP-768).
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