Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

HSP60 (rat), (recombinant)

 
ADI-SPP-742-D 50 µg 201.00 USD
 
ADI-SPP-742-F 200 µg 494.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:Chaperonin 60, CPN60, HspD1, Heat shock protein 60
 
MW:~60kDa
 
Source:Produced in E. coli.
 
UniProt ID:P63039
 
Formulation:Liquid. In 50mM TRIS-HCl, pH 7.5, containing 150mM sodium chloride, 0.1mM PMSF, and 1.0mM DTT.
 
Purity:≥85% (SDS-PAGE; Western blot)
 
Purity Detail:Purified by multi-step chromatography.
 
Applications:WB
Activity assay, in vitro Assay
 
Application Notes:ATPase activity assay (positive). Western blot control.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:Rat Hsp60 belongs to a highly conserved family that includes molecular chaperones from a variety of species such as plant Hsp60 (known as Rubisco binding protein), GroEL, the E. coli Hsp60, and the 65 kDa major antigen of mycobacteria. Hsp60 is localized in the mitochondrial matrix of eukaryotes, and in the chloroplast of plants. Hsp60s from divergent species share a number of common characteristics: high abundance; induction with environmental stress such as heat shock; homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg+ and ATP; ATPase activity; and a role in folding and assembly of oligomeric protein structures. These similarities correspond with studies in which the single-ring human mitochondrial homolog Hsp60 and its co-chaperonin Hsp10 were expressed in anE. coli strain engineered to keep the groE operon under strict regulatory control. The findings demonstrate that expression of Hsp60 -Hsp10 enabled successful performance of all essential in vivo functions of GroEL and its co-chaperonin, GroES. Several studies reveal a possible link between members of the Hsp60 family and a number of autoimmune diseases, atherosclerosis, and chlamydial dise ase. Overexpression of self Hsp60 is seen in the synovial tissue of rheumatoid arthritic (RA) patients , and can accompany both cellular and humoral reactivity against Hsp60 in RA. Chlamydial heat shock protein Hsp60, a homolog ofE. coli GroEL, appears capable of eliciting macrophage activation, and several studies reveal a correlation between Hsp60 responses and the immunopathologic manifestations of human chlamydial disease.
 
SPP-742 SDS-PAGE
SDS-PAGE analysis: Lane 1: MWM, Lane 2: 2.0µg of purified Rat HSP60 Protein (Prod. No. ADI-SPP-742)
SPP-742 WB
Western Blot analysis: Lane 1: MW marker; Lane 2: 100ng Rat HSP60 Protein (Prod. No. ADI-SPP-742); Lane 3: 100ng E. coli GroEL Protein (Prod. No. ADI-SPP-610); Left Blot probed with HSP60 mAb (Prod. No. ADI-SPA-806) at 1.0µg/ml; Right Blot probed with GroEL mAb (Prod. No. ADI-SPS-870) at 1.0µg/ml
Please mouse over
SPP-742 SDS-PAGE SPP-742 WB

Product Literature References

Different heat shock protein 60 species share pro-inflammatory activity but not binding sites on macrophages: H. Kolb, et al. ; FEBS Lett. 533, 105 (2003), Application(s): In Vitro Assay , Abstract;
Different efficiency of heat shock proteins (HSP) to activate human monocytes and dendritic cells: superiority of HSP60: M. Heike, et al. ; J. Immunol. 169, 6141 (2002), Application(s): In Vitro Assay , Abstract;

Related Products

Goat anti-mouse IgG, polyclonal antibody (AP conjugate) 

ELISA, WB | Print as PDF
 
ADI-SAB-101-J 1 ml 129.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
HSP60
By biological activity:
HSP60 Recombinant protein
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Catalogs
Heat Shock Proteins & the Cellular Stress Response Catalog
Heat Shock Proteins & the Cellular Stress Response Catalog
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,