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HO-1 (human), (recombinant)

 
ADI-SPP-732-D 50 µg 201.00 USD
 
ADI-SPP-732-F 200 µg 479.00 USD
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Product Specification

Alternative Name:HMOX1, HSP32, Heat shock protein 32, Heme oxygenase 1
 
Recommended Dilutions/Conditions:Western Blot (100ng)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
MW:~32kDa
 
Source:Produced in E. coli.
 
UniProt ID:P09601
 
Formulation:Liquid. In 50mM TRIS-HCl, pH 7.5, containing 2% glycerol, 450mM sodium chloride, 1mM DTT, and 0.1mM PMSF.
 
Purity:≥90% (SDS-PAGE; Western blot)
 
Purity Detail:Purified by multi-step chromatography.
 
Applications:WB
Antigen microarray
 
Application Notes:Western blot control.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.
 
SPP-732 SDS-PAGE
SDS-PAGE analysis of Prod. No. ADI-SPP-732: Lane 1: MWM, Lane 2: 0.5μg; Lane 3: 1μg; Lane 4: 2μg; Lane 5: 5μg of Prod. No. ADI-SPP-732 detected by Coomassie stain.
SPP-732 WB
Western Blot analysis of Prod. No. ADI-SPP-732: Lane 1: MW marker; Lane 2: 100 ng of Prod. No. ADI-SPP-732 probed with Prod. No. ADI-OSA-110 at 1:1000 dilution.
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SPP-732 SDS-PAGE SPP-732 WB

Product Literature References

The role of heme oxygenase-1 in drug metabolizing dysfunction in the alcoholic fatty liver exposed to ischemic injury: S.W. Park, et al.; Toxicol. Appl. Pharmacol. 292, 30 (2016), Application(s): Western blot, Abstract;
Antigen microarrays identify unique serum autoantibody signatures inclinical and pathologic subtypes of multiple sclerosis: H. Weiner, et al. ; PNAS 105, 18889 (2008), Application(s): Antigen Microarray , Abstract;

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