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GroES (E. coli), (recombinant)

 
ADI-SPP-620-D 50 µg 201.00 USD
 
ADI-SPP-620-F 200 µg 473.00 USD
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Product Specification

Alternative Name:Heat shock protein 10, HSP10, 10kDa Chaperonin, Protein Cpn10 GroES protein
 
MW:~10kDa
 
Source:Produced in E. coli.
 
UniProt ID:P0A6F9 (strain K12)
 
Formulation:Liquid. In 50mM TRIS, pH 7.5, containing 100mM sodium chloride, 10mM magnesium chloride, and 5mM DTT.
 
Purity:≥95% (SDS-PAGE; Western blot)
 
Purity Detail:Purified by multi-step chromatography.
 
Applications:WB
 
Application Notes:Western blot control.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:The E. coli heat shock proteins GroEL and GroES are two of the most thoroughly characterized members of a class of proteins known collectively as "molecular chaperones". GroEL (cpn60; Hsp60 homolog) is an acidic 60kDa E. coli heat shock protein which possesses a weak ATPase activity. In its native form, GroEL exists as a 14-mer consisting of two stacked rings each composed of seven identical subunits of Mr ~57,250. GroES (cpn10; Hsp10 homolog) consists of a single ring of seven identical subunits of Mr ~10,350. Molecular chaperones, such as GroEL and GroES, are thought to function by interacting with transiently exposed interactive surfaces during their nascent synthesis, folding, transport and oligomerization. The net effect of chaperone participation in these processes is a reduction in inappropriate protein-protein interactions that might produce a nonfunctional structure. The GroEL and GroES genes, constituting the GroE operon, are members of the heat shock regulon ofE. coli. Synthesis of the GroE protein, which accounts for approximately 1% of the cellular protein at 37oC, increases to about 10% of the total protein content upon shifting the growth temperature to 46oC.
 
SPP-620 SDS-PAGE
SDS-PAGE analysis: Lane 1: MW marker, Lane 2: 0.5µg, Lane 3: 1µg, Lane 4: 2µg, Lane 5: 5µg GroES
SPP-620 WB
Western Blot analysis: Lane 1: MWM, Lane 2: GroES probed with anti-GroES pAb.
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SPP-620 SDS-PAGE SPP-620 WB

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Heat Shock Proteins & the Cellular Stress Response Catalog
Heat Shock Proteins & the Cellular Stress Response Catalog
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