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Calreticulin (human), (recombinant) (His-tag)

 
ADI-SPP-600-D 50 µg 213.00 USD
 
ADI-SPP-600-F 200 µg 508.00 USD
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Product Specification

Alternative Name:CALR, CRT
 
Recommended Dilutions/Conditions:Western Blot (100 ng, control)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
MW:~59kDa
 
Source:Produced in E. coli. Human calreticulin is fused at the N-terminus to a His-tag.
 
UniProt ID:P27797
 
Formulation:Liquid, In 50mM TRIS, pH 7.5, containing 150mM sodium chloride, 5mM calcium chloride and 5% glycerol.
 
Purity:≥90% (SDS-PAGE; Western blot)
 
Purity Detail:Purified by multi-step chromatography.
 
Applications:WB
 
Application Notes:Western blot control.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:The multifunctional, multi-compartmental protein Calreticulin (Crt) functions as a soluble molecular chaperone of new or misfolded proteins as well as a calcium-binding protein. Most abundant in the ER lumen, Crt expression also occurs in other membrane-bound organelles, the cell surface, and extracellularly. Also known as CRP-55, calregulin and HACBP (high affinity calcium-binding protein). Crt contains the ER retrieval sequence, KDEL, and is the soluble paralog of the ER membrane protein Calnexin (Cnx). Crt’s three domains include a 180 residue N-terminal domain, a proline-rich P-domain (residues 189-288) that binds calcium with high affinity and shares homology with Cnx and calmegin, and a 110 residue C-terminal domain that binds calcium with low affinity but high capacity. The P-domain may interact with the co-chaperone ERp57 (Grp58), a thiol reductase. The NMR structure of the P-domain consists of an extended hairpin that appears to form a curved protrusion from the Crt core domain. Both Crt and its membrane bound homolog CNX interact with proteins and glycoproteins possessing monoglucosylated N-glycans. The Crt/Cnx cycle promotes correct folding, inhibits aggregation of folding intermediates, blocks premature oligomerization, regulates ER degradation, and prevents incompletely folded glycoproteins from exiting to the Golgi complex. Crt also appears to function as an auto-antigen in systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block, and halothane hepatitis. A diversity of additional functions attributed to Crt includes adhesion, blood function, and cardiac and neuronal development gene expression.
 
ADI-SPP-600 SDS-PAGE
SDS-PAGE Analysis of Calreticulin (human), (recombinant) (His-tag). Lane 1: MW Marker; Lane 2: 1µg Calreticulin.
ADI-SPP-600 WB
Western Blot Analysis of Calreticulin (human), (recombinant) (His-tag). Lane 1: MW Marker, Lane 2: HeLa lysate (Prod. No. ADI-LYC-HL100), Lane 3: Calreticulin. Probed with Calreticulin pAb (left) or mAb (right).
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ADI-SPP-600 SDS-PAGE ADI-SPP-600 WB

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