Product Details
| Alternative Name: | SerpinH1, Colligin, Gp46, Heat shock protein 47, Collagen binding protein |
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| MW: | ~47kDa |
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| Source: | Produced in E. coli. |
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| UniProt ID: | P50454 |
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| Formulation: | Liquid. In 30mM TRIS, pH 8.0, containing 100mM sodium chloride, 1mM DTT, and 0.4M arginine. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Purity Detail: | Purified by multi-step chromatography. |
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| Endotoxin Content: | < 50 EU/mg determined by Limulus Amebocyte Lysate (LAL) gel clot assay. |
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| Applications: | WB
Antigen microarray
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| Application Notes: | Western blot control. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Scientific Background: | Hsp47, also known as colligin and gp46, is an ER-resident collagen-specific stress glycoprotein involved in the synthesis and assembly of varoius collagens as a molecular chaperone. Hsp47 is a member of the serpin superfamily, but localizes to the ER via binding of its C-terminal RDEL sequence to KDEL receptors in the ER. Hsp47 binds procollagen co-translationally as it enters the ER, and dissociates prior to its secretion. |
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| Regulatory Status: | RUO - Research Use Only |
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SDS-PAGE Analysis. Lane 1: MW marker, Lane 2: 1.0 µg of purified Hsp47 (Prod. No. ADI-SPP-535)
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Product Literature References
Antigen microarrays identify unique serum autoantibody signatures inclinical and pathologic subtypes of multiple sclerosis: H. Weiner, et al. ; PNAS
105, 18889 (2008),
Application(s): Antigen Microarray ,
Abstract;
