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HSP40/Hdj2 (human), (recombinant)

 
ADI-SPP-405-D 50 µg 201.00 USD
 
ADI-SPP-405-F 200 µg 479.00 USD
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Product Specification

Alternative Name:Heat shock protein 40, DnaJ protein homolog 2
 
Recommended Dilutions/Conditions:Western Blot (100ng, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
MW:~54kDa
 
Source:Produced in E. coli.
 
UniProt ID:P31689
 
Formulation:Liquid. In TBS containing 1mM DTT.
 
Purity:≥90% (SDS-PAGE; Western blot)
 
Purity Detail:Purified by multi-step chromatography.
 
Applications:WB
 
Application Notes:Western blot control.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:Hdj2 is an approximately 40 kDa member of the heat shock protein (HSP40) family, a eukaryotic homolog of the E. coli DnaJ protein. Eukaryotic DnaJ-like proteins share structural domain conservation with DnaJ, namely: 1) an N-terminal ~70 amino acid J domain, 2) a glycine- and phenylalanine-rich domain, 3) a cysteine-rich zinc finger domain, and 4) a poorly conserved C-terminal domain. The human constitutive Hdj2 protein shares full domain conservation with DnaJ, as opposed to the human inducible Hdj1 which lacks the zinc-finger domain. The HSP40 J domain regulates its interaction with HSP70 (E. coli DnaK), increasing HSP70 ATPase activity and in turn enhancing substrate binding by the HSP70 chaperone. HSP40 co-chaperones are involved in nearly all aspects of protein synthesis and secretion because of their importance in HSP70 function, and they are also thought to have an intrinsic ability to bind and fold some misfolded proteins. Hdj2 has been specifically shown to interact with Hsc70, the constitutive form of HSP70, in the biogenesis of the cystic fibrosis transmembrane conductance regulator (CFTR), mutations of which are known to cause inherited cystic fibrosis.
 
SPP-405 SDS-PAGE
SDS-PAGE analysis of HSP40/Hdj2 (human), (recombinant) (Prod. No. ADI-SPP-405): Lane 1: MWM, Lane 2: 0.5μg, Lane 3: 1μg, Lane 4: 2μg, Lane 5: 5μg.
SPP-405 WB
Western Blot analysis: Lane 1: MW marker, Lane 2: Hdj2 probed with anti-Hdj2 mAb.
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SPP-405 SDS-PAGE SPP-405 WB

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