Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

TRAP1 monoclonal antibody (9B6)

 
ADI-SPA-971-0050 50 µg 194.00 USD
 
ADI-SPA-971-0200 200 µg 410.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:HSP75, Mitochondrial HSP90, TNF receptor-associated protein 1, MtHSP90
 
Clone:9B6
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Recombinant human TRAP1.
 
UniProt ID:Q12931
 
Species reactivity:Human, Mouse
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1:500, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~72kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress. It includes cytoplasmic (Hsp90-alpha/beta), ER (Grp94) and mitochondrial (TRAP1) localized members. Hsp90 is structurally composed of an N-terminal ATP binding domain, a medial substrate-binding region, and a C-terminal dimerization motif.  Hsp90 dimers function in cooperation with cochaperones to stabilize a multitude of client protein substrates. TRAP1 (aka Hsp75) was originally identified as a chaperone binding partner for retinoblastoma protein. TRAP1 was later found by immunofluorescence to be localized to the mitochondria. TRAP1 is significantly more active as an ATPase due in part to the lack of regulatory elements in the C-terminus, but retains the ability to dimerize. However, it does not form stable complexes with typical cochaperones of Hsp90 like p23 and HOP4. Recently TRAP1 has been identified as a protective element for cell survival, and has been suggested as a novel target for anti-cancer therapeutics.
 
ADI-SPA-971 WB
Western blot analysis of TRAP1 probed with TRAP1, mAb (9B6) (Prod. No. ADI-SPA-971): Lane 1: MWM, Lane 2: TRAP1 Recombinant Human Protein (Prod. No. ADI-SPP-848), Lane 3: HL-60 Cell Lysate, Lane 4: Hep G2 Cell Lysate, Lane 5: 3T3 Cell Lysate, Heat Shocked (Prod. No. ADI-LYC-3T101), Lane 6: HeLa Cell Lysate, Heat Shocked (Prod. No. ADI-LYC-HL101)
Please mouse over
ADI-SPA-971 WB

General Literature References

Cytoprotective mitochondrial chaperone TRAP-1 as a novel molecular target in localized and metastatic prostate cancer: I. Leav, et al.; Am. J. Pathol. 176, 393 (2010), Abstract;
TRAP1, a novel mitochondrial chaperone responsible for multi-drug resistance and protection from apoptotis in human colorectal carcinoma cells: E. Costantino, et al.; Cancer Lett. 279, 39 (2009), Abstract;
Regulation of heat shock protein 90 ATPase activity by sequences in the carboxyl terminus: B.A. Owen, et al.; J. Biol. Chem. 277, 7086 (2002), Abstract;
The hsp90-related protein TRAP1 is a mitochondrial protein with distinct functional properties: S.J. Felts, et al.; J. Biol. Chem. 275, 3305 (2000), Abstract;
The charged region of Hsp90 modulates the function of the N-terminal domain: T. Scheibel, et al.; PNAS U.S.A. 96, 1297 (1999), Abstract;
Oligomeric forms of the 90-kDa heat shock protein: T. Nemoto & N. Sato; Biochem. J. 330, 989 (1998), Abstract;
A new member of the hsp90 family of molecular chaperones interacts with the retinoblastoma protein during mitosis and after heat shock: C.F. Chen, et al.; Mol. Cell. Biol. 1, 4691 (1996), Abstract;

Related Products

TRAP1 polyclonal antibody 

WB | Print as PDF
 
ADI-SPA-970-0050 50 µg 151.00 USD
 
ADI-SPA-970-0200 200 µg 330.00 USD
Do you need bulk/larger quantities?
 

TRAP1 (human), (recombinant) (His-tag) 

Produced in E. coli. Human TRAP1 (aa 60-704) is fused at the N-terminus to a His-tag., ≥95% (SDS-PAGE; Western blot), WB | Print as PDF
 
ADI-SPP-848-050 50 µg 208.00 USD
 
ADI-SPP-848-200 200 µg 494.00 USD
Do you need bulk/larger quantities?
 

3T3 (heat shocked) , (cell lysate) 

Mouse, WB | Print as PDF
 
ADI-LYC-3T101-F 200 µg 109.00 USD
Do you need bulk/larger quantities?
 

HeLa (heat shocked) , (cell lysate) 

Human, WB | Print as PDF
 
ADI-LYC-HL101-F 200 µg 109.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
TRAP1
By biological activity:
TRAP1 Monoclonal antibody
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Catalogs
Heat Shock Proteins & the Cellular Stress Response Catalog
Heat Shock Proteins & the Cellular Stress Response Catalog
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,