HSF1, mAb (10H8)

Alternative Name:	heat shock factor

Purity Detail:	Protein G affinity purified.

Formulation:	Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Clone:	10H8

Isotype:	Rat IgG1

Immunogen:	Recombinant mouse HSF1.

Specificity:	Recognizes human, mouse, rat, monkey and rabbit HSF1. Predicted MW of ~60kDa. Detects a band of ~85kDa by Western blot.

Application:	Immunohistochemistry (paraffin sections) Western Blot (1:1000, colorimetric) Optimal conditions must be determined individually for each application.

Long Term Storage:	-20°C

Miscellaneous/General:	HSFs (Heat Shock family of transcription factors), which consists of HSF 1-4, bind to highly conserved Heat shock elements (HSEs) in the promoter regions of heat shock genes, ultimately regulating the expression of Heat shock proteins (Hsps). On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleoplasmic distribution.