[pSer³²⁶]HSF1, pAb



ADI-SPA-902-D	50 µg	190.00 USD

ADI-SPA-902-F	200 µg	375.00 USD

Product Specification

Purity Detail:	Protein A affinity purified.

Formulation:	Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Immunogen:	Synthetic peptide corresponding to the N-terminus of human HSF1 phosphorylated at Ser ³²⁶.

Source/Host:	From rabbit.

Specificity:	Recognizes human HSF1. Predicted MW of ~60kDa. Detects a band of 85-95kDa by Western blot depending on hyperphosphorylation of the native protein.

Application:	Western Blot (1:1000, ECL) Optimal conditions must be determined individually for each application.

Long Term Storage:	-20°C

Miscellaneous/General:	HSFs (Heat Shock family of transcription factors), which consists of HSF 1-4, bind to highly conserved Heat shock elements (HSEs) in the promoter regions of heat shock genes, ultimately regulating the expression of Heat shock proteins (Hsps). On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleoplasmic distribution.

Western blot analysis of HSF1 (pSer326): Lane 1: MW marker, Lane 2: HeLa, Lane 3: HeLa, Heat-Shocked (No recovery time), probed with HSF1 (pSer326) pAb.

Western Blot analysis of Recombinant HSF1 and HSF1 (phospho): Lane 1: MWM, Lane 2: Recombinant HSF1, Lane 3: Recombinant HSF1 (phospho), probed with HSF1 (pSer326) pAb.

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