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HSF1 polyclonal antibody

 
ADI-SPA-901-D 50 µg 169.00 USD
 
ADI-SPA-901-F 200 µg 374.00 USD
 
ADI-SPA-901-J 1 mg 1,475.00 USD
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Product Specification

Alternative Name:HSTF1, Heat shock factor protein 1
 
Host:Rabbit
 
Immunogen:Recombinant human HSF1.
 
UniProt ID:Q00613
 
GenBank ID:M64673
 
Source:Purified from rabbit serum.
 
Species reactivity:Human, Mouse, Rat
Gerbil, Monkey, Rabbit
 
Applications:ELISA, IP, WB
EMSA
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects bands ~80 to ~95kDa (depending on phosphorylation status) by Western blot analysis.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:HSFs (Heat Shock family of transcription factors), which consists of HSF 1-4, bind to highly conserved Heat shock elements (HSEs) in the promoter regions of heat shock genes, ultimately regulating the expression of Heat shock proteins (Hsps). On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleoplasmic distribution.
 
Technical Info/Product Notes:ADI-SPA-901 is tested against HeLa and HeLa Heat-Shocked lysates. Upon heat shock, HSF1 is hyperphosphorylated. ADI-SPA-901 recognizes both phosphorylated and non-phosporylated HSF1 in Western Blot.
 
HSF1 polyclonal antibody Western blot
Western blot analysis of HSF1: Lane 1: Molecular Weight Marker, Lane 2: HSF1 (human), (recombinant) (Prod. No. ADI-SPP-900), Lane 3: HeLa Cell Lysate (Prod. No. ADI-LYC-HL100), Lane 4: HeLa Cell Lysate (Heat Shocked) (Prod. No. ADI-LYC-HL101), Lane 5: 3T3 Cell Lysate (Prod. No. ADI-LYC-3T100), Lane 6: PC-12 Cell Lysate (Prod. No. ADI-LYC-PC100), Lane 7: RK-13 Cell Lysate, Lane 8: Vero Cell Lysate
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HSF1 polyclonal antibody Western blot

Product Literature References

Histone deacetylase inhibitor SAHA treatment prevents the development of heart failure after myocardial infarction via an induction of heat-shock proteins in rats: S. Nagata, et al.; Biol. Pharm. Bull. 42, 453 (2019), Abstract;
IER family proteins are regulators of protein phosphatase PP2A and modulate the phosphorylation status of CDC25A: T. Ueda, et al.; Cell. Signal. 55, 81 (2019), Abstract;
A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods 15, 909 (2018), Abstract;
Induction of suppressor of cytokine signaling 3 via HSF-1-HSP70-TLR4 axis attenuates neuroinflammation and ameliorates postoperative pain: Y.X. Fan, et al.; Brain Behav. Immun. 68, 111 (2018), Abstract;
mTORC2/AKT/HSF1/HuR constitute a feed-forward loop regulating Rictor expression and tumor growth in glioblastoma: B. Holmes, et al.; Oncogene 37, 732 (2018), Abstract; Full Text
zHSF1 modulates zper2 expression in zebrafish embryos: L. Mennetrier, et al.; Chronobiol. Int. 6, 1 (2018), Abstract;
Transcriptional regulatory logic of the diurnal cycle in the mouse liver: J.A. Sobel, et al.; PLoS Biol. 15, e2001069 (2017), Abstract; Full Text
BIIB021, a synthetic Hsp90 inhibitor, induces mutant ataxin-1 degradation through the activation of heat shock factor 1: Y. Ding, et al.; Neuroscience 327, 20 (2016), Application(s): Western blot, Abstract;
ChIP - Does it work correctly? The optimization steps of chromatin immunoprecipitation: M. Kus-Liskiewicz; Acta Biol. Hung. 67, 373 (2016), Abstract;
Effects of intrinsic aerobic capacity, aging and voluntary running on skeletal muscle sirtuins and heat shock proteins: S. Karvinen, et al.; Exp. Gerontol. 79, 46 (2016), Application(s): Immunoblotting, Abstract;
The Helicobacter pylori cytotoxin CagA is essential for suppressing host heat shock protein expression: B.J. Lang, et al.; Cell Stress Chaperones 21, 523 (2016), Abstract; Full Text
Active heat shock transcription factor 1 supports migration of the melanoma cells via vinculin down-regulation: A. Tomo-Jonik, et al.; Cell. Signal. 27, 394 (2015), Abstract;
Cross talk between cytokine and hyperthermia-induced pathways: identification of different subsets of NF-κB-dependent genes regulated by TNFα and heat shock: P. Janus, et al.; Mol. Genet. Genomics 290, 1979 (2015), Abstract; Full Text
Disruption of polyubiquitin gene Ubc leads to attenuated resistance against arsenite-induced toxicity in mouse embryonic fibroblasts: M.N. Kim, et al.; Biochim. Biophys. Acta 1853, 996 (2015), Application(s): Western Blotting, Abstract;
Lysine Deacetylases Regulate the Heat Shock Response Including the Age-Associated Impairment of HSF1: E. Zelin, et al.; J. Mol. Biol. 427, 1644 (2015), Abstract; Full Text
NZ28-induced inhibition of HSF1, SP1 and NF-κB triggers the loss of the natural killer cell-activating ligands MICA/B on human tumor cells: D. Schilling, et al.; Cancer Immunol. Immunother. 64, 599 (2015), Abstract; Full Text
Thiopental protects human neuroblastoma cells from apoptotic cell death — Potential role of heat shock protein 70: M. Roesslein, et al.; Life Sci. 139, 40 (2015), Application(s): Western blot, Abstract;
Crosstalk between HSF1 and HSF2 during the heat shock response in mouse testes: J. Korfanty, et al.; Int. J. Biochem. Cell Biol. 57, 76 (2014), Abstract;
Differential translocation of heat shock factor-1 after mild and severe stress to human skin fibroblasts undergoing aging in vitro: D. Demirovic, et al.; J. Cell Commun. Signal. 8, 333 (2014), Abstract; Full Text
HSF1 deficiency and impaired HSP90-dependent protein folding are hallmarks of aneuploid human cells: N. Donnelly, et al.; EMBO J. 33, 2374 (2014), Abstract; Full Text
Novel isoforms of heat shock transcription factor 1, HSF1γα and HSF1γβ, regulate chaperone protein gene transcription: A. Neueder, et al.; J. Biol. Chem. 289, 19894 (2014), Abstract; Full Text
Cytotoxicity of withaferin A in glioblastomas involves induction of an oxidative stress-mediated heat shock response while altering Akt/mTOR and MAPK signaling pathways: P.T. Grogan, et al.; Invest. New Drugs 31, 545 (2013), Abstract; Full Text
Dynamics of the Full Length and Mutated Heat Shock Factor 1 in Human Cells: G. Herbomel, et al.; PLoS One 8, e67566 (2013), Abstract; Full Text
OLA1 protects cells in heat shock by stabilizing HSP70: R.F. Mao, et al.; Cell Death Dis. 14, e491 (2013), Abstract; Full Text
Overexpression of heat shock transcription factor 1 enhances the resistance of melanoma cells to doxorubicin and paclitaxel: N. Vydra, et al.; BMC Cancer 13, 504 (2013), Abstract; Full Text
The SIRT1 modulators AROS and DBC1 regulate HSF1 activity and the heat shock response: R. Raynes, et al.; PLoS One 8, e54364 (2013), Abstract; Full Text
Expression of heat shock transcription factor 1 and its downstream target protein T-cell death associated gene 51 in the spinal cord of a mouse model of amyotrophic lateral sclerosis: T. Mimoto, et al.; Brain Res. 1488, 123 (2012), Application(s): Immunohistochemistry and western-blotting using mouse neurons, Abstract;
Chemical and biological approaches synergize to ameliorate protein-folding diseases: T.W. Mu, et al. ; Cell 134, 769 (2008), Application(s): WB using human cell lysates, Abstract;
Postinsult treatment with lithium reduces brain damage and facilitates neurological recovery in a rat ischemia/reperfusion model: D.M. Chuang, et al. ; PNAS 100, 6210 (2003), Application(s): EIA using rat samples, Abstract;
TNFalpha mediates susceptibility to heat-induced apoptosis by protein phosphatase-mediated inhibition of the HSF1/hsp70 stress response: G. Steiner, et al. ; Cell Death Differ. 10, 1126 (2003), Application(s): EMSA using human samples, Abstract;
17-beta-estradiol induces heat shock proteins in brain arteries and potentiates ischemic heat shock protein induction in glia and neurons: F.R. Sharp, et al. ; J. Cereb. Blood Flow Metab. 22, 183 (2002), Application(s): IHC using rat & gerbil samples, Abstract;
Evidence for a mechanism of repression of heat shock factor 1 transcriptional activity by a multichaperone complex: R. Voellmy, et al. ; J. Biol. Chem. 276, 45791 (2001), Application(s): IP using human samples, Abstract;
Role of cyclopentenone prostaglandins in rat carrageenin pleurisy: M. Di Rosa, et al. ; FEBS Lett. 508, 61 (2001), Application(s): EMSA, WB using rat samples, Abstract;
Signal transducer and activator of transcription-1 and heat shock factor-1 interact and activate the transcription of the Hsp-70 and Hsp-90 beta gene promoters: D.S. Latchman, et al. ; J. Biol. Chem. 274, 1723 (1999), Application(s): IP using human samples, Abstract;

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