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HSF1 polyclonal antibody

ADI-SPA-901-D 50 µg 169.00 USD
ADI-SPA-901-F 200 µg 374.00 USD
ADI-SPA-901-J 1 mg 1,475.00 USD
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Product Specification

Alternative Name:HSTF1, Heat shock factor protein 1
Immunogen:Recombinant human HSF1.
UniProt ID:Q00613
GenBank ID:M64673
Species reactivity:Human, Mouse, Rat
Gerbil, Monkey, Rabbit
Applications:ELISA, IP, WB
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects bands ~80 to ~95kDa (depending on phosphorylation status) by Western blot analysis.
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:HSFs (Heat Shock family of transcription factors), which consists of HSF 1-4, bind to highly conserved Heat shock elements (HSEs) in the promoter regions of heat shock genes, ultimately regulating the expression of Heat shock proteins (Hsps). On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleoplasmic distribution.
Technical Info/Product Notes:ADI-SPA-901 is tested against HeLa and HeLa Heat-Shocked lysates. Upon heat shock, HSF1 is hyperphosphorylated. ADI-SPA-901 recognizes both phosphorylated and non-phosporylated HSF1 in Western Blot.
Western blot analysis of HSF1: Lane 1: Molecular Weight Marker, Lane 2: HSF1 (human), (recombinant) (Prod. No. ADI-SPP-900), Lane 3: HeLa Cell Lysate (Prod. No. ADI-LYC-HL100), Lane 4: HeLa Cell Lysate (Heat Shocked) (Prod. No. ADI-LYC-HL101), Lane 5: 3T3 Cell Lysate (Prod. No. ADI-LYC-3T100), Lane 6: PC-12 Cell Lysate (Prod. No. ADI-LYC-PC100), Lane 7: RK-13 Cell Lysate, Lane 8: Vero Cell Lysate
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Product Literature References

A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods 15, 909 (2018), Abstract;
Induction of suppressor of cytokine signaling 3 via HSF-1-HSP70-TLR4 axis attenuates neuroinflammation and ameliorates postoperative pain: Y.X. Fan, et al.; Brain Behav. Immun. 68, 111 (2018), Abstract;
zHSF1 modulates zper2 expression in zebrafish embryos: L. Mennetrier, et al.; Chronobiol. Int. 6, 1 (2018), Abstract;
BIIB021, a synthetic Hsp90 inhibitor, induces mutant ataxin-1 degradation through the activation of heat shock factor 1: Y. Ding, et al.; Neuroscience 327, 20 (2016), Application(s): Western blot, Abstract;
ChIP - Does it work correctly? The optimization steps of chromatin immunoprecipitation: M. Kus-Liskiewicz; Acta Biol. Hung. 67, 373 (2016), Abstract;
Effects of intrinsic aerobic capacity, aging and voluntary running on skeletal muscle sirtuins and heat shock proteins: S. Karvinen, et al.; Exp. Gerontol. 79, 46 (2016), Application(s): Immunoblotting, Abstract;
Disruption of polyubiquitin gene Ubc leads to attenuated resistance against arsenite-induced toxicity in mouse embryonic fibroblasts: M.N. Kim, et al.; Biochim. Biophys. Acta 1853, 996 (2015), Application(s): Western Blotting, Abstract;
Thiopental protects human neuroblastoma cells from apoptotic cell death — Potential role of heat shock protein 70: M. Roesslein, et al.; Life Sci. 139, 40 (2015), Application(s): Western blot, Abstract;
Expression of heat shock transcription factor 1 and its downstream target protein T-cell death associated gene 51 in the spinal cord of a mouse model of amyotrophic lateral sclerosis: T. Mimoto, et al.; Brain Res. 1488, 123 (2012), Application(s): Immunohistochemistry and western-blotting using mouse neurons, Abstract;
Chemical and biological approaches synergize to ameliorate protein-folding diseases: T.W. Mu, et al. ; Cell 134, 769 (2008), Application(s): WB using human cell lysates, Abstract;
Postinsult treatment with lithium reduces brain damage and facilitates neurological recovery in a rat ischemia/reperfusion model: D.M. Chuang, et al. ; PNAS 100, 6210 (2003), Application(s): EIA using rat samples, Abstract;
TNFalpha mediates susceptibility to heat-induced apoptosis by protein phosphatase-mediated inhibition of the HSF1/hsp70 stress response: G. Steiner, et al. ; Cell Death Differ. 10, 1126 (2003), Application(s): EMSA using human samples, Abstract;
17-beta-estradiol induces heat shock proteins in brain arteries and potentiates ischemic heat shock protein induction in glia and neurons: F.R. Sharp, et al. ; J. Cereb. Blood Flow Metab. 22, 183 (2002), Application(s): IHC using rat & gerbil samples, Abstract;
Evidence for a mechanism of repression of heat shock factor 1 transcriptional activity by a multichaperone complex: R. Voellmy, et al. ; J. Biol. Chem. 276, 45791 (2001), Application(s): IP using human samples, Abstract;
Role of cyclopentenone prostaglandins in rat carrageenin pleurisy: M. Di Rosa, et al. ; FEBS Lett. 508, 61 (2001), Application(s): EMSA, WB using rat samples, Abstract;
Signal transducer and activator of transcription-1 and heat shock factor-1 interact and activate the transcription of the Hsp-70 and Hsp-90 beta gene promoters: D.S. Latchman, et al. ; J. Biol. Chem. 274, 1723 (1999), Application(s): IP using human samples, Abstract;

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