HSF1, pAb



ADI-SPA-901-D	50 µg	130.00 USD

ADI-SPA-901-F	200 µg	310.00 USD

ADI-SPA-901-J	1 mg	1'255.00 USD

Product Specification

Alternative Name:	heat shock factor

Purity Detail:	Protein A affinity purified.

Formulation:	Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Immunogen:	Recombinant human HSF1.

Source/Host:	From rabbit.

Specificity:	Recognizes human, mouse, rat, gerbil, monkey and rabbit HSF1. Detects a band of ~95kDa by Western blot.

Application:	EIA Electrophoretic Mobility Shift Assays Immunoprecipitation Western Blot (1:1000, colorimetric) Optimal conditions must be determined individually for each application.

Long Term Storage:	-20°C

Miscellaneous/General:	HSFs (Heat Shock family of transcription factors), which consists of HSF 1-4, bind to highly conserved Heat shock elements (HSEs) in the promoter regions of heat shock genes, ultimately regulating the expression of Heat shock proteins (Hsps). On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleoplasmic distribution.

Western blot analysis of HSF1: Lane 1: Molecular Weight Marker, Lane 2: HSF1 (human), (recombinant) (Prod. No. ADI-SPP-900), Lane 3: HeLa Cell Lysate (Prod. No. ADI-LYC-HL100), Lane 4: HeLa Cell Lysate (Heat Shocked) (Prod. No. ADI-LYC-HL101), Lane 5: 3T3 Cell Lysate (Prod. No. ADI-LYC-3T100), Lane 6: PC-12 Cell Lysate (Prod. No. ADI-LYC-PC100), Lane 7: RK-13 Cell Lysate, Lane 8: Vero Cell Lysate

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Product Literature References

Chemical and biological approaches synergize to ameliorate protein-folding diseases: T.W. Mu, et al. ; Cell 134, 769 (2008), Application(s): WB using human cell lysates, Abstract;

Postinsult treatment with lithium reduces brain damage and facilitates neurological recovery in a rat ischemia/reperfusion model: D.M. Chuang, et al. ; PNAS 100, 6210 (2003), Application(s): EIA using rat samples, Abstract;

TNFalpha mediates susceptibility to heat-induced apoptosis by protein phosphatase-mediated inhibition of the HSF1/hsp70 stress response: G. Steiner, et al. ; Cell Death Differ. 10, 1126 (2003), Application(s): EMSA using human samples, Abstract;

17-beta-estradiol induces heat shock proteins in brain arteries and potentiates ischemic heat shock protein induction in glia and neurons: F.R. Sharp, et al. ; J. Cereb. Blood Flow Metab. 22, 183 (2002), Application(s): WB using gerbil samples, Abstract;

Evidence for a mechanism of repression of heat shock factor 1 transcriptional activity by a multichaperone complex: R. Voellmy, et al. ; J. Biol. Chem. 276, 45791 (2001), Application(s): IP using human samples, Abstract;

Role of cyclopentenone prostaglandins in rat carrageenin pleurisy: M. Di Rosa, et al. ; FEBS Lett. 508, 61 (2001), Application(s): EMSA, WB using rat samples, Abstract;

Signal transducer and activator of transcription-1 and heat shock factor-1 interact and activate the transcription of the Hsp-70 and Hsp-90 beta gene promoters: D.S. Latchman, et al. ; J. Biol. Chem. 274, 1723 (1999), Application(s): IP using human samples, Abstract;