HMOX1, Hsp32, Heat shock protein 32, Heme oxygenase 1
Host:
Rabbit
Immunogen:
Synthetic peptide corresponding to the sequence near the N-terminus of human HO-1.
UniProt ID:
P09601
GenBank ID:
X14782
Gene/Protein Identifier:
3162 (Entrez GeneID), 141250 (OMIM)
Source:
Purified from rabbit serum.
Species reactivity:
Human, Mouse, Rat Dog, Hamster, Monkey, Rabbit
Applications:
IHC (PS), IP, WB
Recommended Dilutions/Conditions:
Immunoprecipitation (1:100) Western Blot (1:1,000, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Application Notes:
Detects a band of ~32kDa by Western blot.
Purity Detail:
Protein A affinity purified.
Formulation:
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Handling:
Avoid freeze/thaw cycles.
Shipping:
Shipped on Blue Ice
Long Term Storage:
-20°C
Scientific Background:
Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.
Immunohistochemistry analysis of human spleen tissue stained with HO-1, pAb at 10µg/ml.
Western blot analysis of HO-1 (HSP32) pAb: Lane 1: HO-1 (HSP32) Recombinant Human Protein, Lane 2:HO-2 Recombinant Human Protein (negative control), Lane 3: Human Liver Microsome Extract, Lane 4: Dog Liver Microsome Extract, Lane 5: Rat Liver Microsome Extract, Lane 6: Mouse Liver Microsome Extract.
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Product Literature References
AICAR, an AMPK activator, protects against cisplatin-induced acute kidney injury through the JAK/STAT/SOCS pathway: B. Tsogbadrakh, et al.; Biochem. Biophys. Res. Commun. 509, 680 (2019), Abstract;
Induction of decay accelerating factor and membrane cofactor protein by resveratrol attenuates complement deposition in human coronary artery endothelial cells: M.G. Detsika, et al.; Biochem. Biophys. Rep. 19, 100652 (2019), Abstract; Full Text
Melatonin prevents early pituitary dysfunction induced by sucrose-rich diets: M.E. Mercau, et al.; J. Pineal Res. 66, e12545 (2019), Abstract;
Oxidized hemoglobin forms contribute to NLRP3 inflammasome-driven IL-1β production upon intravascular hemolysis: B.B. Nyakundi, et al.; Biochim. Biophys. Acta Mol. Basis Dis. 1865, 464 (2019), Abstract;
Candida albicans β-glucan-containing particles increase HO-1 expression in oral keratinocytes via ROS/p38MAPK/Nrf2 pathway: Y. Ishida, et al.; Infect. Immun. 86, e00575 (2018), Abstract;
Differential Effects of Antiretroviral Drugs on Neurons In Vitro: Roles for Oxidative Stress and Integrated Stress Response: A.L. Stern, et al.; J. Neuroimmune Pharmacol. 13, 64 (2018), Abstract;
Identification of Novel Protein Targets of Dimethyl Fumarate Modification in Neurons and Astrocytes Reveals Actions Independent of Nrf2 Stabilization: G.G. Piroli, et al.; Mol. Cell. Proteomics (2018), Abstract;
Lutzomyia longipalpis Saliva Induces Heme Oxygenase-1 Expression at Bite Sites: N.F. Luz, et al.; Front. Immunol. 9, 2779 (2018), Abstract; Full Text
Myeloid HO-1 modulates macrophage polarization and protects against ischemia-reperfusion injury: M. Zhang, et al.; JCI Insight 3, e120596 (2018), Abstract;
Nano-targeted induction of dual ferroptotic mechanisms eradicates high-risk neuroblastoma: B. Hassannia, et al.; J. Clin. Invest. 128, 3341 (2018), Abstract; Full Text
Propofol induces nuclear localization of Nrf2 under conditions of oxidative stress in cardiac H9c2 cells: T. Shinjo, et al.; PLoS One 13, e0196191 (2018), Application(s): WB w/ rat cardiac myoblast cells, Abstract; Full Text
MicroRNA-1304 suppresses human non-small cell lung cancer cell growth in vitro by targeting heme oxygenase-1: C.G. Li, et al.; Acta Pharmacol. Sin. 38, 110 (2017), Abstract; Full Text
Molecular hydrogen protects against oxidative stress-induced SH-SY5Y neuroblastoma cell death through the process of mitohormesis: Y. Murakami, et al.; PLoS One 12, e0176992 (2017), Abstract; Full Text
Pathological significance and prognostic implications of heme oxygenase 1 expression in non-muscle-invasive bladder cancer: Correlation with cell proliferation, angiogenesis, lymphangiogenesis and expression of VEGFs and COX-2: T. Matsuo, et al.; Oncol. Lett. 13, 275 (2017), Abstract; Full Text
Resveratrol inhibits release of soluble fms-like tyrosine kinase (sFlt-1) and soluble endoglin and improves vascular dysfunction – implications as a preeclampsia treatment: N.J. Hannan, et al.; Sci. Rep. 7, 1819 (2017), Abstract; Full Text
Toxicity Evaluation of Graphene Oxide and Titania Loaded Nafion Membranes in Zebrafish: R. Pecoraro, et al.; Front. Physiol. 8, 1039 (2017), Abstract; Full Text
Violet/blue light activates Nrf2 signaling and modulates the inflammatory response of THP-1 monocytes: L.A. Trotter, et al.; Photochem. Photobiol. Sci. 16, 883 (2017), Abstract;
Iron gene expression profile in atherogenic Mox macrophages: L. Marques, et al.; Biochim. Biophys. Acta 1862, 1137 (2016), Application(s): Western blot, Abstract;
Isolation of basal membrane proteins from BeWo cells and their expression in placentas from fetal growth-restricted pregnancies: S.Y. Oh, et al.; Placenta 39, 24 (2016), Application(s): Western blot, Abstract;
Moderate exercise prevents functional remodeling of the anterior pituitary gland in diet-induced insulin resistance in rats: role of oxidative stress and autophagy: M.E. Mercau, et al.; Endocrinology 157, 1135 (2016), Application(s): Western blot, Abstract; Full Text
Omeprazole induces heme oxygenase-1 in fetal human pulmonary microvascular endothelial cells via hydrogen peroxide-independent Nrf2 signaling pathway: A. Patel, et al.; Toxicol. Appl. Pharmacol. 311, 26 (2016), Application(s): Western blot, whole-cell protein extracts , Abstract;
Partial contribution of the Keap1-Nrf2 system to cadmium-mediated metallothionein expression in vascular endothelial cells: Y. Shinkai, et al.; Toxicol. Appl. Pharmacol. 395, 37 (2016), Application(s): Western blot, Abstract;
Vascular and angiogenic activities of CORM-401, an oxidant-sensitive CO-releasing molecule: S. Fayad-Kobeissi, et al.; Biochem. Pharmacol. 102, 64 (2016), Application(s): Western blot, Abstract;
Vitamin C promotes wound healing through novel pleiotropic mechanisms: B.M. Mohammed, et al.; Int. Wound J. 13, 572 (2016), Abstract;
Activated AMPK boosts the Nrf2/HO-1 signaling axis—A role for the unfolded protein response: K. Zimmermann, et al.; Free Radic. Biol. Med. 88, 417 (2015), Abstract; Full Text
Gedunin Binds to MD-2 and Impairs LPS-Induced TLR4 Signaling in Macrophages: P.V. Borges, et al.; Mol. Pharmacol. 88, 949 (2015), Application(s): Western Blot, Abstract; Full Text
Methylglyoxal, the foe and friend of glyoxalase and Trx/TrxR systems in HT22 nerve cells: A.L. Dafre, et al.; Free Radic. Biol. Med. 89, 8 (2015), Application(s): Western Blot, Abstract;
Neuron-specific antioxidant OXR1 extends survival of a mouse model of amyotrophic lateral sclerosis: K.X. Liu, et al.; Brain 138, 1167 (2015), Application(s): Immunoprecipitation, Western Blotting, Abstract; Full Text
Nuclear Factor, Erythroid 2-Like 2 Regulates Expression of Type 3 Inositol 1,4,5-Trisphosphate Receptor and Calcium Signaling in Cholangiocytes: J. Weerachayaphorn, et al.; Gastroenterology 149, 211 (2015), Abstract; Full Text
Peroxisome proliferator activated receptor gamma loaded dental implant improves osteogenesis of rat mandible: G. Bhattarai, et al.; J. Biomed. Mater. Res. B Appl. Biomater. 103, 587 (2015), Abstract;
Heme oxygenase-1 expression is associated with tumor aggressiveness and outcomes in patients with bladder cancer: a correlation with smoking intensity: Y. Miyata, et al.; Transl. Res. 164, 468 (2014), Abstract;
Microphthalmia-associated transcription factor as the molecular target of cadmium toxicity in human melanocytes: W. Chantarawong, et al.; Biochem. Biophys. Res. Commun. 454, 594 (2014), Abstract;
Nuclear heme oxygenase-1 (HO-1) modulates subcellular distribution and activation of Nrf2, impacting metabolic and anti-oxidant defenses: C. Biswas, et al.; J. Biol. Chem. 289, 26882 (2014), Abstract; Full Text
Combined losartan and nitro-oleic acid remarkably improves diabetic nephropathy in mice: Y. Liu, et al.; Am. J. Physiol. Renal Physiol. 305, F1555 (2013), Abstract; Full Text
Neuroprotective role of superoxide dismutase 1 in retinal ganglion cells and inner nuclear layer cells against N-methyl-d-aspartate-induced cytotoxicity: K. Yuki, et al.; Exp. Eye Res. 115, 230 (2013), Abstract;
Early outcome in renal transplantation from large donors to small and size-matched recipients - a porcine experimental model: K. Ravlo, et al.; Pediatr. Transplant. 16, 599 (2012), Abstract;
Regional and systemic hemodynamic responses following the creation of a murine arteriovenous fistula: L. Kang, et al.; Am. J. Physiol. Renal Physiol. 301, F845 (2011), Application(s): WB using mouse tissue homogenates, Abstract; Full Text
Inhaled carbon monoxide reduces leukocytosis in a murine model of sickle cell disease: G. Vercellotti, et al. ; Am. J. Physiol. Heart Circ. Physiol. 297, H1243 (2009), Application(s): WB using mouse tissue, Abstract;
The p38 Mitogen-Activated Protein Kinase Pathway Is Involved in theRegulation of Heme Oxygenase-1 by Acidic Extracellular pH in AorticSmooth Muscle Cells: H. Christou, et al. ; J. Cell. Biochem. 105, 1298 (2008), Application(s): WB using rat tissue culture, Abstract;
Mechanism of ascididemin-induced cytotoxicity: L.R. Barrows, et al. ; Chem. Res. Toxicol. 16, 113 (2003), Application(s): WB using rat samples, Abstract;
Enhanced splenomegaly and severe liver inflammation in haptoglobin/hemopexin double-null mice after acute hemolysis: E. Tolosano, et al.; Blood 100, 4201 (2002), Application(s): IHC, WB using mouse samples, Abstract;
Heme oxygenase-1-derived carbon monoxide requires the activation of transcription factor NF-kappa B to protect endothelial cells from tumor necrosis factor-alpha -mediated apoptosis: M.P. Soares, et al. ; J. Biol. Chem. 277, 17950 (2002), Application(s): WB using rat samples, Abstract;
Selection of potential therapeutics based on in vivo spatiotemporal transcription patterns of heme oxygenase-1: C.H. Contag, et al. ; J. Mol. Med. 80, 655 (2002), Application(s): WB using mouse samples, Abstract;
