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HO-1 polyclonal antibody

 
ADI-SPA-895-D 50 µg 168.00 USD
 
ADI-SPA-895-F 200 µg 363.00 USD
 
ADI-SPA-895-J 1 mg 1,530.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: ALX-210-116

Product Specification

Alternative Name:HMOX1, Hsp32, Heat shock protein 32, Heme oxygenase 1
 
Host:Rabbit
 
Immunogen:Recombinant rat HO-1 (Hsp32) lacking the membrane spanning region.
 
UniProt ID:P06762
 
GenBank ID:J02722
 
Species reactivity:Human, Mouse, Rat
Dog, Rabbit, Sheep
 
Applications:Flow Cytometry, ICC, IHC (PS), WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~32kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.
 
HO-1 polyclonal antibody Immunohistochemistry
Immunohistochemistry analysis of human spleen tissue stained with HO-1, pAb at 10µg/ml.
HO-1 polyclonal antibody Immunohistochemistry
HO-1 polyclonal antibody Structure
Crystal structure of human HO-1 (Hsp32) in complex with its substrate heme.
HO-1 polyclonal antibody Western blot
Western blot analysis of HO-1 pAb: Lane 1: MW Marker, Lane 2: HO-1 (rat), Recombinant Protein, Lane 3: Human Liver Microsomes, Lane 4: Mouse Liver Microsomes, Lane 5: Rat Liver Microsomes, Lane 6: Canine Liver Microsomes.
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HO-1 polyclonal antibody Immunohistochemistry HO-1 polyclonal antibody Immunohistochemistry HO-1 polyclonal antibody Structure HO-1 polyclonal antibody Western blot

Product Literature References

Metallothionein 3 Is a Hypoxia-Upregulated Oncogene Enhancing Cell Invasion and Tumorigenesis in Human Bladder Carcinoma Cells: K. Tsui, et al.; Int. J. Mol. Sci. 20, E980 (2019), Application(s): used in antiserum for Immunoblot Assay with bladder transitional carcinoma cell lines, Abstract; Full Text
Rosmarinic acid protects against MPTP-induced toxicity and inhibits iron-induced α-synuclein aggregation: L. Qu, et al.; Neuropharmacology 144, 291 (2019), Abstract;
A Central Role for Heme Oxygenase-1 in the Control of Intestinal Epithelial Chemokine Expression: J.C. Onyiah, et al.; J. Innate Immun. 10, 228 (2018), Abstract;
Arachidonic Acid Induces ARE/Nrf2-Dependent Heme Oxygenase-1 Transcription in Rat Brain Astrocytes: C.C. Lin, et al.; Mol. Neurobiol. 55, 3328 (2018), Abstract;
Elevated Nrf-2 responses are insufficient to mitigate protein carbonylation in hepatospecific PTEN deletion mice: D.R. Petersen, et al.; PLoS One 13, e0198139 (2018), Abstract; Full Text
Ferritin H Deficiency in Myeloid Compartments Dysregulates Host Energy Metabolism and Increases Susceptibility to Mycobacterium tuberculosis Infection: V.P. Reddy, et al.; Front. Immunol. 9, 869 (2018), Abstract; Full Text
Haem oxygenase-1 up-regulation by rosiglitazone via ROS-dependent Nrf2-antioxidant response elements axis or PPARγ attenuates LPS-mediated lung inflammation: R.L. Cho, et al.; Br. J. Pharmacol. 175, 3928 (2018), Abstract;
Heme oxygenase-1 induction by rosiglitazone via PKCα/AMPKα/p38 MAPKα/SIRT1/PPARγ pathway suppresses lipopolysaccharide-mediated pulmonary inflammation: R.L. Cho, et al.; Biochem. Pharmacol. 148, 222 (2018), Abstract;
Microanatomic Distribution of Myeloid Heme Oxygenase-1 Protects against Free Radical-Mediated Immunopathology in Human Tuberculosis: K.C. Chinta, et al.; Cell Rep. 25, 1938 (2018), Abstract;
Neuroprotective effect of an Nrf2-ARE activator identified from a chemical library on dopaminergic neurons: Y. Izumi, et al.; Eur. J. Pharmacol. 818, 470 (2018), Abstract;
Nrf2 Activation Provides Atheroprotection in Diabetic Mice Through Concerted Upregulation of Antioxidant, Anti-inflammatory, and Autophagy Mechanisms: I. Lazaro, et al.; Front. Pharmacol. 9, 819 (2018), Abstract; Full Text
Revisiting the putative role of heme as a trigger of inflammation: F. Vallelian, et al.; Pharmacol. Res. Perspect. 6, e00392 (2018), Abstract; Full Text
Role of TLR4 signaling in the nephrotoxicity of heme and heme proteins: K.A. Nath, et al.; Am. J. Physiol. Renal Physiol. 314, F906 (2018), Abstract;
Sulforaphane protects rabbit corneas against oxidative stress injury in keratoconus through activation of the Nrf-2/HO-1 antioxidant pathway: R. Liu, et al.; Int. J. Mol. Med. 42, 2315 (2018), Abstract; Full Text
Various roles of heme oxygenase-1 in response of bone marrow macrophages to RANKL and in the early stage of osteoclastogenesis: U. Florczyk-Soluch, et al.; Sci. Rep. 8, 10797 (2018), Abstract; Full Text
Alternative activation-skewed microglia/macrophages promote hematoma resolution in experimental intracerebral hemorrhage: C.F. Chang, et al.; Neurobiol. Dis. 103, 54 (2017), Abstract; Full Text
Atractylenolide I restores HO-1 expression and inhibits Ox-LDL-induced VSMCs proliferation, migration and inflammatory responses in vitro: W. Li, et al.; Exp. Cell Res. 353, 26 (2017), Abstract;
Carbon monoxide does not contribute to vascular tonus improvement in exercise-trained rats with chronic nitric oxide synthase inhibition: S.N. Ulker, et al.; Nitric Oxide 65, 60 (2017), Abstract;
Differential inhibition of rat and mouse microsome heme oxygenase by derivatives of imidazole and benzimidazole: M. Hum, et al.; Can. J. Physiol. Pharmacol. 95, 1454 (2017), Abstract;
Enzymatically synthesized glycogen inhibits colitis through decreasing oxidative stress: T. Mitani, et al.; Free Radic. Biol. Med. 106, 355 (2017), Abstract;
Fluvastatin inhibits AGE-induced cell proliferation and migration via an ERK5-dependent Nrf2 pathway in vascular smooth muscle cells: A.R. Hwang, et al.; PLoS One 12, e0178278 (2017), Abstract; Full Text
Heme Oxygenase-1 Induction by Carbon Monoxide Releasing Molecule-3 Suppresses Interleukin-1β-Mediated Neuroinflammation: C.C. Lin, et al.; Front. Mol. Neurosci. 10, 387 (2017), Abstract; Full Text
Hemopexin counteracts systolic dysfunction induced by heme-driven oxidative stress: G. Ingoglia, et al.; Free Radic. Biol. Med. 108, 452 (2017), Application(s): Western Blot, Abstract;
Inflammatory signature of cerebellar neurodegeneration during neonatal hyperbilirubinemia in Ugt1 -/- mouse model: S. Vodret, et al.; J. Neuroinflammation 14, 64 (2017), Abstract; Full Text
Neuroprotective effects of 2,3,5,4'-tetrahydoxystilbene-2-O-β-D-glucoside from Polygonum multiflorum against glutamate-induced oxidative toxicity in HT22 cells: S.Y. Lee, et al.; J. Ethnopharmacol. 195, 64 (2017), Abstract;
Time-dependent change of in vivo optical imaging of oxidative stress in a mouse stroke model: Y. Nakano, et al.; J. Neurosci. Res. 95, 2030 (2017), Abstract; Full Text
A potent Nrf2 activator, dh404, bolsters antioxidant capacity in glial cells and attenuates ischaemic retinopathy: D. Deliyanti, et al.; Clin. Sci. 130, 1375 (2016), Abstract;
Attenuated heme oxygenase-1 responses predispose the elderly to pulmonary nontuberculous mycobacterial infections: R. Surolia, et al.; Am. J. Physiol. Lung Cell. Mol. Physiol. 311, L928 (2016), Abstract; Full Text
Copper diethyldithiocarbamate as an activator of Nrf2 in cultured vascular endothelial cells: T. Fujie, et al.; J. Biol. Inorg. Chem. 21, 263 (2016), Abstract; Full Text
Epigallocatechin-3-gallate protects against hepatic ischaemia-reperfusion injury by reducing oxidative stress and apoptotic cell death: E. Tak, et al.; J. Int. Med. Res. 44, 1248 (2016), Application(s): Western blot, Abstract; Full Text
Extracellular Vesicles from Metastatic Rat Prostate Tumors Prime the Normal Prostate Tissue to Facilitate Tumor Growth: S. Halin Bergström, et al.; Sci. Rep. 6, 31085 (2016), Application(s): Immunohistochemistry, morphological analysis, rat prostate tissue sections, Abstract; Full Text
Extratumoral Heme Oxygenase-1 (HO-1) Expressing Macrophages Likely Promote Primary and Metastatic Prostate Tumor Growth: S. Halin Bergstrom, et al.; PLoS One 11, e0157280 (2016), Abstract; Full Text
Fisetin stimulates autophagic degradation of phosphorylated tau via the activation of TFEB and Nrf2 transcription factors: S. Kim, et al.; Sci. Rep. 6, 24933 (2016), Application(s): Cell culture, Abstract;
Haem oxygenase 1 expression is associated with prognosis in cholangiocarcinoma patients and with drug sensitivity in xenografted mice: S. Kongpetch, et al.; Cell Prolif. 49, 90 (2016), Abstract;
Haemoxygenase modulates cytokine induced neutrophil chemoattractant in hepatic ischemia reperfusion injury: N. Tapuria, et al.; World J. Gastroenterol. 22, 7518 (2016), Application(s): Western blot for HO-1, frozen liver tissue samples, Abstract; Full Text
Haptoglobin increases the vulnerability of CD163-expressing neurons to hemoglobin: J. Chen-Roetling, et al.; J. Neurochem. 139, 586 (2016), Abstract; Full Text
Impairment of biliverdin reductase-A promotes brain insulin resistance in Alzheimer disease: A new paradigm: E. Barone, et al.; Free Radic. Biol. Med. 91, 127 (2016), Application(s): Western blot, Abstract;
MicroRNA-27b Enhances the Hepatic Regenerative Properties of Adipose-Derived Mesenchymal Stem Cells: K.D. Chen, et al.; Mol. Ther. Nucleic Acids 5, e285 (2016), Application(s): Western blot, Abstract;
Modulation of Melanogenesis by Heme Oxygenase-1 via p53 in Normal Human Melanocytes: H.S. Lim, et al.; Chonnam. Med. J. 52, 45 (2016), Application(s): Western blot, Abstract; Full Text
Protective effects of monomethyl fumarate at the inflamed blood-brain barrier: J.L. Lim, et al.; Microvasc. Res. 105, 61 (2016), Application(s): Western blot, Abstract;
Sanguiin H-6, a constituent of Rubus parvifolius L., inhibits receptor activator of nuclear factor-κB ligand-induced osteoclastogenesis and bone resorption in vitro and prevents tumor necrosis factor-α-induced osteoclast formation in vivo: E. Sakai, et al.; Phytomedicine 23, 828 (2016), Application(s): Decreases ROS production, Abstract;
SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2: H. Pan, et al.; Cell Res. 16, 190 (2016), Application(s): Western blot, Abstract;
Transcriptional Induction of Metallothionein by Tris(pentafluorophenyl)stibane in Cultured Bovine Aortic Endothelial Cells: T. Fujie, et al.; Int. J. Mol. Sci. 17, 1381 (2016), Application(s): Western blot analysis of protein expression, Abstract; Full Text
Cerebroprotection of Flavanol (-)-Epicatechin after Traumatic Brain Injury via Nrf2-dependent and -independent Pathways: T. Cheng, et al.; Free Radic. Biol. Med. 92, 15 (2015), Application(s): Western blot analysis, Abstract;
CO Induces Nrf2-Dependent Heme Oxygenase-1 Transcription by Cooperating with Sp1 and c-Jun in Rat Brain Astrocytes: P.L. Chi, et al.; Mol. Neurobiol. 52, 277 (2015), Abstract;
Cytoprotective responses in HaCaT keratinocytes exposed to high doses of curcumin: D.M. Lundvig, et al.; Exp. Cell Res. 336, 298 (2015), Application(s): Western Blot, Abstract;
Hemoglobin induces monocyte recruitment and CD163-macrophage polarization in abdominal aortic aneurysm: A. Subio-Navarro, et al.; Int. J. Cardiol. 201, 66 (2015), Application(s): Immunohistochemical analysis, Abstract;
Inhibition of dipeptidyl peptidase IV protects tacrolimus-induced kidney injury: S. Lim, et al.; Lab. Invest. 95, 1174 (2015), Application(s): Immunoblot analysis, Abstract;
Novel synthetic pyridyl analogues of CDDO-Imidazolide are useful new tools in cancer prevention: M. Cao, et al. ; Pharmacol. Res. 100, 135 (2015), Application(s): Western Blot, Abstract;
Role of nuclear factor-erythroid 2-related factor 2 (NRF2) in the transcriptional regulation of brain ABC transporters during acute acetaminophen (APAP) intoxication in mice: C.I. Ghanem, et al.; Biochem. Pharmacol. 94, 203 (2015), Application(s): Western Blotting, Abstract;
Ascorbic acid deficiency decreases hepatic cytochrome P-450, especially CYP2B1/2B2, and simultaneously induces heme oxygenase-1 gene expression in scurvy-prone ODS rats: M. Kobayashi, et al.; Biosci. Biotechnol. Biochem. 78, 1060 (2014), Abstract;
Heme oxygenase-1 overexpression exacerbates heart failure with aging and pressure overload but is protective against isoproterenol-induced cardiomyopathy in mice: M.A. Allwood, et al.; Cardiovasc. Pathol. 23, 231 (2014), Abstract;
Neurochemical phenotype of cytoglobin-expressing neurons in the rat hippocampus: C.A. Hundahl, et al.; Biomed. Rep. 2, 620 (2014), Application(s): IHC using rat tissue, Abstract; Full Text
NRF2 immunolocalization in human breast cancer patients as a prognostic factor: Y. Onodera, et al.; Endocr. Relat. Cancer 21, 241 (2014), Abstract;
Preventive effect of agaro-oligosaccharides on non-steroidal anti-inflammatory drug-induced small intestinal injury in mice: Y. Higashimura, et al.; J. Gastroenterol. Hepatol. 29, 310 (2014), Abstract;
Systemic hemin therapy attenuates blood-brain barrier disruption after intracerebral hemorrhage: X. Lu, et al.; Neurobiol. Dis. 70, 245 (2014), Abstract;
Distinct effects of methamphetamine on autophagy-lysosome and ubiquitin-proteasome systems in HL-1 cultured mouse atrial cardiomyocytes: I. Funakoshi-Hirose, et al.; Toxicology 312, 74 (2013), Abstract;
Pleural mesothelial cell differentiation and invasion in fibrogenic lung injury: J.S. Zolak, et al.; Am. J. Pathol. 182, 1239 (2013), Application(s): WB using mouse cell lysates, Abstract; Full Text
Crucial role of heme oxygenase-1 on the sensitivity of cholangiocarcinoma cells to chemotherapeutic agents: S. Kongpetch, et al.; PLoS One 7, e34994 (2012), Application(s): WB using human cell lysates, Abstract; Full Text
Enhanced expression of heme oxygenase-1 and carbon monoxide excitatory effects in oxytocin and vasopressin neurones during water deprivation: W.L. Reis, et al.; J. Neuroendocrinol. 24, 653 (2012), Application(s): IHC using rat tissue, Abstract; Full Text
Heme oxygenase-1 regulates the immune response to influenza virus infection and vaccination in aged mice: N.W. Cummins, et al.; FASEB J. 26, 2911 (2012), Application(s): Western Blotting, Abstract; Full Text
Increased production of superoxide anion contributes to dysfunction of the arteriovenous fistula: M.V. Tsapenko, et al.; Am. J. Physiol. Renal Physiol. 303, F1601 (2012), Application(s): IHC using rat tissue, Abstract; Full Text
Regional and systemic hemodynamic responses following the creation of a murine arteriovenous fistula: L. Kang, et al.; Am. J. Physiol. Renal Physiol. 301, F845 (2011), Application(s): IHC using mouse tissue, Abstract; Full Text
Adaptive HNE-Nrf2-HO-1 pathway against oxidative stress is associated with acute gastric mucosal lesions: T. Ueyama, et al. ; Am. J. Physiol. Gastrointest. Liver Physiol. 295, G460 (2008), Application(s): WB, IHC using rat cell lysates & tissue, Abstract;
Induction of Heme Oxygenase-1 and Ferritin in the Kidney in Warm Antibody Hemolytic Anemia: F. Fervenza, et al. ; Am. J. Kidney Dis. 52, 972 (2008), Application(s): IHC using human tissue, Abstract;
Induction of Heme Oxygenase-1 Is a Beneficial Response in a Murine Model of Venous Thrombosis: K. Nath, et al. ; Am. J. Pathol. 173, 1882 (2008), Application(s): IHC using mouse tissue, Abstract;
Comparison of the heme-free and -bound crystal structures of human heme oxygenase-1: L. Lad, et al.; J. Biol. Chem. 278, 78 (2003), (ref. 6), Abstract;
Cytochrome P450 2E1 null mice provide novel protection against cisplatin-induced nephrotoxicity and apoptosis: H. Liu, et al.; Kidney Int. 63, 1687 (2003), Abstract;
Red blood cells upregulate cytoprotective proteins and the labile iron pool in dividing human T cells despite a reduction in oxidative stress: F.A. Arosa, et al. ; Free Radic. Biol. Med. 35, 1404 (2003), Application(s): Flow using human samples, Abstract;
Red blood cells upregulate cytoprotective proteins and the labile iron pool in dividing human T cells despite a reduction in oxidative stress: A.M. Fonseca, et al.; Free Radic. Biol. Med. 35, 1404 (2003), (ref. 7), Abstract;
Role of heme oxygenase-1 protein in the neuroprotective effects of cyclopentenone prostaglandin derivatives under oxidative stress: Y. Watanabe, et al. ; Eur. J. Neurosci. 17, 2249 (2003), Application(s): ICC, WB using mouse samples, Abstract;
Role of heme oxygenase-1 protein in the neuroprotective effects of cyclopentenone prostaglandin derivatives under oxidative stress: T. Satoh, et al.; Eur. J. Neurosci. 17, 2249 (2003), (ref. 8), Abstract;
Glucose deprivation induces heme oxygenase-1 gene expression by a pathway independent of the unfolded protein response: A. Agarwal, et al. ; J. Biol. Chem. 277, 1933 (2002), Application(s): WB using human samples, Abstract;
Regulation and localization of HSP70 and HSP25 in the kidney of rats undergoing long-term administration of angiotensin II: N. Ishizaka, et al.; Hypertension 39, 122 (2002), (ref. 4), Abstract;
Oxidative stress and induction of heme oxygenase-1 in the kidney in sickle cell disease: R.P. Hebbel, et al. ; Am. J. Pathol. 158, 893 (2001), Application(s): ICC using human & rat samples, Abstract;
Induction of heme oxygenase-1 modulates cis-aconitase activity in lens epithelial cells: U.P. Andley, et al. ; Biochem. Biophys. Res. Commun. 270, 324 (2000), Application(s): WB using rabbit samples, Abstract;
Induction of heme oxygenase-1 modulates cis-aconitase activity in lens epithelial cells: D.M. Rzymkiewicz, et al.; BBRC 270, 324 (2000), (ref. 9), Abstract;
Heme oxygenase: recent advances in understanding its regulation and role: K.K. Elbirt & H.L. Bonkovsky; Proc. Assoc. Am. Physicians 111, 438 (1999), (ref. 1), Abstract;
Peroxynitrite induces haem oxygenase-1 in vascular endothelial cells: a link to apoptosis: R. Foresti, et al.; Biochem. J. 339, 729 (1999), (ref. 3), Abstract;
Expression pattern of heme oxygenase isoenzymes 1 and 2 in normal and stress-exposed rat liver: M. Bauer, et al. ; Hepatology 27, 829 (1998), Application(s): Flow using rat samples, Abstract;
Carbon monoxide formation in the ductus arteriosus in the lamb: implications for the regulation of muscle tone: C. Ackerley, et al. ; Br. J. Pharmacol. 120, 599 (1997), Application(s): IHC using sheep samples, Abstract;
Carbon monoxide formation in the ductus arteriosus in the lamb: implications for the regulation of muscle tone: F. Coceani, et al.; Br. J. Pharmacol. 120, 599 (1997), (ref. 10), Abstract;
Expression of heme oxygenase-1 (HSP32) in human prostate: normal, hyperplastic, and tumor tissue distribution: M.D. Maines & P.A. Abrahamsson; Urology 47, 727 (1996), (ref. 2), Abstract;
Heme oxygenase-1 is associated with the neurofibrillary pathology of Alzheimer's disease: M.A. Smith, et al.; Am. J. Pathol. 145, 42 (1994), (ref. 5), Abstract;

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